FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1162925
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACGGCCGTGTCCTCGGCTCCCAGGGTGTTCATTTGCAGATGCA34630.2977836059935077No Hit
GTATAATGACTGGCCTCCGAAAATCACTTTCGGCCCTGGGACCAAAGTGG31510.2709547047316035No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA30250.2601199561450652No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29940.25745426403250427No Hit
GTCATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT29480.2534987208977363No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT27940.24025625040307846No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23400.20121675946428189No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22230.19115592149106778No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20660.17765548079196852No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20000.171980136294258No Hit
GTTTATTACTGTGCGAAATGTATGATAACAGTACCTGGGGTGAATGACGC19480.1675086527506073No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGGAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT18960.1630371692069566No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATTCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA18500.15908162607218868No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATGACTGG18110.15572801341445064No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17990.15469613259668508No Hit
CTCCTGTGCAGCCTCTGGATTCACCCTTAGCAGTCATGCCGTGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG17980.15461014252853797No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17600.15134251993894704No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC17410.14970870864415162No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17060.1466990562590021No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT16790.1443773244190296No Hit
CCCTTAGCAGTCATGCCGTGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG16240.1396478706709375No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16160.13895995012576046No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT16140.13878796998946621No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16100.1384440097168777No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC16030.1378420792398478No Hit
GTCTCCAGCCACCCTGTCTTTTTCTCCAGGGGAAAGAGCCGCCCTCTCCT15900.13672420835393512No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15830.1361222778769052No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15760.1355203473998753No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15590.1340585162413741No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGAATACCAGTGGCCCTGGT15120.13001698303845907No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGATTTTCGGAGG14890.12803921147107508No Hit
CTGGTATTCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT14710.12649139024442677No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14660.12606143990369112No Hit
GTTTATTACTGTCAGCAGTATAATGACTGGCCTCCGAAAATCACTTTCGGCCCTGGGACCAAAGTGGATATCAAA14290.12287980738224734No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14050.12081604574671624No Hit
CCATTGTCCCTTGGCCCCAGATATCAAAAGCGTCATTCACCCCAGGTACT13860.1191822344519208No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13840.11901025431562653No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13800.11866629404303802No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAGCGTCATTCA13780.11849431390674377No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13780.11849431390674377No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGT13550.1165165423393598No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13490.11600060193047704No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA13420.11539867145344712No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT13400.11522669131715288No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13380.11505471118085861No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13360.11488273104456437No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC13290.11428080056753445No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT13240.11385085022679882No Hit
GAAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTTTTTCTCCAGG13040.11213104886385622No Hit
GAATACCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG12820.11023926736461939No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12750.10963733688758948No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT12740.10955134681944236No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12720.10937936668314809No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT12690.10912139647870671No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12650.1087774362061182No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG12620.1085194660016768No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12420.10679966463873422No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12420.10679966463873422No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12310.10585377388911581No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12300.10576778382096867No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGATTTTCGGAGGCCAGTCATTATA12240.10525184341208589No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC12220.10507986327579165No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGAATACCA12150.10447793279876173No Hit
GGACAATGGTCACCGTCTCTTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGC11990.10310209170840767No Hit
CCCCAGGTACTGTTATCATACATTTCGCACAGTAATAAACGGCCGTGTCC11840.10181224068620073No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT11640.10009243932325818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTGC354.143658E-969.0356941
TAGCGCC200.00609976354.2648765
CCGATGG400.006908691552.5796170
CGCGGTT352.3047976E-549.16963239
GGTATCA9450.048.2492031
CTCTTAT2050.046.475211
GTGGTAT2050.044.8153761
CTTATAC8900.043.5844961
TATACAC8850.043.4443863
TTATACA9050.043.238082
TGGTATC2100.042.1280362
CATCGCG1750.041.15033336
CTACGCT9200.041.10496528
GCATCGC1750.041.10223435
TGCATCG3300.040.453234
ATCGCGG856.91216E-1140.38722237
GTATCAA25250.040.023241
TACGCTG9500.039.83092529
ACGAGAC9400.039.8148122
CCCACGA9500.039.3923219
Produced by FastQC (version 0.11.5)