FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1162925
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77630.6675408990261625No Hit
GTATAATGACTGGCCTCCGAAAATCACTTTCGGCCCTGGGACCAAAGTGG32350.2781778704559623No Hit
GTAATAAACGGCCGTGTCCTCGGCTCCCAGGGTGTTCATTTGCAGATGCA29800.2562504030784445No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29330.2522088698755294No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA26900.231313283315777No Hit
GTCATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT26870.23105531311133562No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT23450.20164670980501753No Hit
CTCCTGTGCAGCCTCTGGATTCACCCTTAGCAGTCATGCCGTGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG19100.1642410301610164No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18580.1597695466173657No Hit
GTTTATTACTGTGCGAAATGTATGATAACAGTACCTGGGGTGAATGACGC18520.15925360620848292No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGGAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT18270.15710385450480469No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18250.15693187436851042No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18230.15675989423221617No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17810.15314831137003676No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATGACTGG17580.1511705398026528No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16810.14454930455532386No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16610.14282950319238127No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16430.14128168196573296No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATTCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA16160.13895995012576046No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15900.13672420835393512No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT15870.13646623814949374No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGAATACCAGTGGCCCTGGT15360.13208074467399017No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15100.1298450029021648No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC14990.12889911215254637No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC14970.12872713201625213No Hit
CTGGTATTCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT14660.12606143990369112No Hit
CCCTTAGCAGTCATGCCGTGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG14650.12597544983554398No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT13910.11961218479265645No Hit
GTCTCCAGCCACCCTGTCTTTTTCTCCAGGGGAAAGAGCCGCCCTCTCCT13700.11780639336156674No Hit
GTTTATTACTGTCAGCAGTATAATGACTGGCCTCCGAAAATCACTTTCGGCCCTGGGACCAAAGTGGATATCAAA13550.1165165423393598No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13370.11496872111271149No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13220.11367887009050455No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13200.11350688995421028No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13190.11342089988606316No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13000.11178708859126772No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGAATACCA12820.11023926736461939No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12740.10955134681944236No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA12600.10834748586538254No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGATTTTCGGAGG12580.1081755057290883No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12530.10774555538835265No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12500.10748758518391126No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAGCGTCATTCA12290.10568179375282155No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12250.10533783348023304No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGT11960.1028441215039663No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11840.10181224068620073No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11840.10181224068620073No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11760.10112432014102372No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGATTTTCGGAGGCCAGTCATTATA11680.10043639959584669No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG200.0069344652.53355450
TACTTCG200.00778097251.0253525
GTGGTAT3200.046.7067951
TGGTATC3250.045.978332
GTATCAA27000.040.007431
GGTATCA9650.039.0726621
TCTGGAG13700.036.58339770
TATCAAC30500.035.297452
TCGAAAT400.002667132834.3112336
TTCGAAA400.002687666634.25760735
ATCAACG32450.033.1663483
TCAACGC32900.032.3933644
GACAGGT2950.032.241361
CAACGCA33250.032.151695
AACGCAG33750.031.7759286
CGCAGAG35550.030.2624878
GCAGAGT36200.029.6253459
AGAGTAC36300.029.4477111
GGTCTAA500.00838036727.1503166
ACGGGTC1156.612936E-826.5600955
Produced by FastQC (version 0.11.5)