FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834409
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23420.280677701223261No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT22760.27276791118024857No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22650.27144961283974645No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20200.24208751343765467No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18860.2260282427442657No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17980.21548185602024905No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT17880.21428340298342896No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16870.20217902731154624No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG15270.1830037787224251No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15100.18096640855983098No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14840.17785043066409878No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14750.17677182293096072No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT14570.17461460746468457No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG14510.17389553564259252No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13230.15855533677129563No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC12790.1532821434092873No Hit
GCTACACACTGGCCGCCGGAGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT10640.12751540311765575No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10600.1270360219029277No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC10560.12655664068819966No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA10450.1252383423476976No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10280.12320097218510348No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10080.12080406611146331No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG9390.11253474015740482No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9270.11109659651322075No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9050.10845999983221657No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8990.10774092801012453No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8940.10714170149171448No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8820.10570355784753041No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC8770.10510433132912038No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG8730.10462495011439234No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8690.10414556889966431No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8580.10282727055916223No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8560.10258757995179824No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8540.10234788934443421No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGCG150.002569490867.5019214
AGCCGAC200.00671440752.9622651
GGTATCA15650.042.924271
ACTAGCG2100.041.92507627
GTGGTAT4050.041.6753431
TCGTATG1350.040.8405640
CTAGCGC2200.040.04346528
GTATCAA39150.039.318531
CGTATGC1600.038.8283941
GACTAGC2300.038.25182326
TAGCGCT2450.035.98987629
TGGTATC4550.035.611812
TATACAC2600.035.0553743
CCCACGA2700.033.76714719
TCTCCGA2700.033.7509611
CGTAACT502.1171245E-433.7509612
GCGTAAC502.1171245E-433.7509611
GAGACTA2800.032.5963524
ACGAGAC2800.032.58070822
TATCAAC47550.032.372672
Produced by FastQC (version 0.11.5)