FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621711
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGG41020.6597920898938574No Hit
GTAATAAGCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG40130.6454767568854338No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC19610.3154198655002083No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19350.31123785810448906No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTCAGGCTGTCATCCCATG17720.28501988866209543No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17150.2758516416791725No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15780.25381567963249807No Hit
GTCTGACACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG14740.23708765004962112No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTCAGG14400.23161887114752674No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT13860.22293316347949452No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG13150.21151306636041506No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13110.21086968060722747No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13020.20942206266255542No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC13000.20910036978596167No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12840.20652682677321132No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12360.19880619773496047No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG12360.19880619773496047No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA12080.19430249746264744No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC11910.19156810801160024No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC11510.1851342504797245No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11440.18400832541164625No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10940.17596600349680155No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10930.17580515705850466No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10700.17210568897767614No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG10660.17146230322448855No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10250.1648675992543159No Hit
CCCCTGAGGGCCGCTGATAATTCCTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTATA9990.16068559185859668No Hit
GTGTCAGACTGGGAGCCAGAGAATCGGCCAGGGACCCCTGAGGGCCGCTG9750.15682527733947124No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9730.15650358446287746No Hit
GTGTATTACTGTGTGAGAGATCTTCGGCGACATTGTAGTAGTGCCAGCTGCTATGGGGACCTTGACTACTGGGGC9700.15602104514798676No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA9670.15553850583309609No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT9610.15457342720331474No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA9520.15312580925864266No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9260.14894380186292344No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC9140.14701364460336072No Hit
GCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG9110.14653110528847005No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8980.14444010159061044No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8960.14411840871401665No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8920.14347502296082906No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC8890.1429924836459384No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT8860.14250994433104772No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC8820.14186655857786012No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8410.13527185460768748No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8170.13141154008856204No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8130.13076815433537448No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8130.13076815433537448No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC7850.12626445406306144No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGCTTATTACTGTGCAG7810.12562106830987388No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC7780.1251385289949832No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT7730.12433429680349874No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC7680.12353006461201425No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7630.1227258324205298No Hit
CCTCAGGGGTCCCTGGCCGATTCTCTGGCTCCCAGTCTGACACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT7600.12224329310563911No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC7550.12143906091415466No Hit
CTGATGGCCAGGGAGGCTGAGGTGTCAGACTGGGAGCCAGAGAATCGGCC7440.11966975009288883No Hit
GGATGACAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC7400.11902636433970125No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7220.11613112845035717No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA7190.11564858913546648No Hit
TTACTGTGCAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCGGAG7140.114844356943982No Hit
GTATACATAATTACTTCCGATGTTGGAGTTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGA7010.1127533532461224No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT6960.11194912105463792No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG6960.11194912105463792No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA6910.11114488886315346No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC6820.10969727091848143No Hit
GGATGAGGCTGCTTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGACCAAG6730.10824965297380937No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC6680.10744542078232491No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG6620.10648034215254354No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC6550.10535441708446529No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC6460.10390679913979325No Hit
CCCAACCACTCAGGCTGTCATCCCATGCTGCACAGTAATAAGCAGCCTCATCCTCGGACCGGAGCCCACTGATGG6430.10342425982490257No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTCAGGC6400.1029417205100119No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTATACATAATTACTT6360.10229833475682432No Hit
GTATTACTGTGTGAGAGATCTTCGGCGACATTGTAGTAGTGCCAGCTGCT6340.10197664188023052No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC6340.10197664188023052No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG6320.10165494900363674No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA6250.10052902393555849No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT6250.10052902393555849No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTACC150.00248948868.040551
AGACCGG355.209906E-663.1922169
CAACTAT200.006825951452.7404853
AGGGGGG1900.052.17967670
GTCCCAT200.007776231551.030411
CAATACT200.007778705651.0263024
TGGTATC1300.049.717942
CGTATGC1250.049.66044641
ACGAGAC1900.048.3251622
GATCTCG1500.047.97967536
GTGGTAT1350.047.8803861
GACATCT1950.047.1391326
CTTATAC1950.047.1049961
TCTCCGA1950.047.08605211
GGTATCA5100.046.027431
AGACATC2000.045.93845725
TCGTATG1200.045.89579840
CCCACGA2100.045.3421319
ATGCCGT1300.045.26004444
TATACAC2200.044.84133
Produced by FastQC (version 0.11.5)