FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2889788
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT121480.42037685809478065No Hit
CTATAGGTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTG118240.4091649629661414No Hit
AACCTATAGTGTTGACAGTAATAAGTTGCAAGATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC112730.39009782032453594No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC100270.3469804705397074No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC95250.3296089540132356No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC83650.2894676010835397No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT68900.23842579455655571No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG65110.2253106456252154No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC65030.22503380870845888No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC61540.21295679821495556No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA60510.2093925229117153No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT59810.20697019989009574No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAGTCATTTAGCCTGGTATCAGCAAAAA58930.203924993805774No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG57260.19814602316848154No Hit
GATTTATCCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA56810.19658881551172613No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT55020.1903945894992989No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG54730.18939105567605652No Hit
GGGCATTAGCAGTCATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATTTATCCTGCATC52430.18143199431930646No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC52270.18087832048579341No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT51360.17772930055768799No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT51010.1765181390468782No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT50490.17471869908796078No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT49990.1729884683582325No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT49760.17219256222255752No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG48790.16883591460688466No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG46520.1609806670939183No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG46250.16004634249986505No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG45560.15765862409284004No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG45440.15724336871770525No Hit
GGATAAATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGC44940.15551313798797697No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG44060.1524679319036552No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA42840.14824616892311823No Hit
ACTTATTACTGTCAACACTATAGGTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT42200.14603147358906607No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT41270.14281324443177146No Hit
CATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGAT40370.1396988291182606No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA39980.13834924914907254No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA39770.13762255224258665No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA39390.13630757688799316No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG38890.13457734615826492No Hit
GTAATAAGTTGCAAGATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG38300.13253567389718554No Hit
CCTATAGTGTTGACAGTAATAAGTTGCAAGATCTTCAGGCTGCAGGCTGC37660.13032097856313335No Hit
ACCTTGATGGGACCCCACTTTGCAAAGTGGATGCAGGATAAATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTG35470.12274256796692352No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC35330.1222581033625996No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATCTTGCAACTTATTACTGTCAACACTATAGGTTCGGC35320.12222349874800505No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT34740.12021643110152025No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC34140.11814015422584632No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC33930.11741345731936044No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33820.11703280655882024No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC33030.11429904200584956No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC33020.114264437391255No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA32860.11371076355774196No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT32720.11322629895341803No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32600.11281104357828324No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA32500.11246499743233761No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC32290.11173830052585171No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC32080.11101160361936585No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA31440.10879690828531367No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC31300.10831244368098975No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT30980.10720509601396366No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATCTTG30710.1062707714199104No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT30450.10537105144045168No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT30430.10530184221126257No Hit
GTCTCCTACAGATGCAGACAGGAAGGATGGAGACTGGGTCAACTGGATGTCACATCTGGCACCTGGGAGCCAGAG29970.10371002993991255No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC29700.10277570534585928No Hit
GTGGATGCAGGATAAATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAATGACTGCTAATG29550.1022566361269408No Hit
GGCTAAATGACTGCTAATGCCCTGACTGGCCCGGCAAGTGATGGTGACTC29300.10139152076207666No Hit
GTCGTGTCCCTTGGCCGAACCTATAGTGTTGACAGTAATAAGTTGCAAGA29290.10135691614748209No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC29000.10035338232423971No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAATCG250.006401038353.60931469
GTGGTAT8550.040.7889181
GTATCAA48800.032.7486341
CGATTGC852.9454222E-629.94151163
AATGGTA7150.029.11851770
AACGCAG58200.027.6037376
ATCAACG58550.027.1597713
TCAACGC58650.027.047814
CAACGCA59250.026.8862255
TATCAAC59750.026.7409782
TCGTTAA2208.1587314E-726.10625870
ACGCAGA61750.026.0168027
CGCAGAG63100.025.5137848
AGAGTAC63600.025.31145111
CAGAGTA64900.024.85741210
GCAGAGT65200.024.692029
TCTGGAG15600.023.47052870
ACTTTAT6350.022.9029796
GTCGCTG8900.022.4456941
TGCACCG10050.022.2113576
Produced by FastQC (version 0.11.5)