FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences932158
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44430.4766359351097132No Hit
GTCCTTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC40530.4347975343235803No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39640.4252497967082834No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30630.3285923630972432No Hit
GTATAGAGATGGATACAACTATTTGAATTGGTACCTGCAGAAGCCAGGGCAGCCTCCACAGGTCCTGATCTATTT29700.3186155136790115No Hit
TCCAAGGACCTTGTAGAACTTGCATGCAGTAATAAACCCCAACATCCTCA29540.3168990664672727No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29150.31271522638865945No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27390.29383430705953284No Hit
GTAATAAACCCCAACATCCTCAGGCCCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA26190.280960952971492No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25330.271735049208396No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24190.2595053628247572No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23810.25542880069687757No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGTAGGTCTAGTCAGAGTCTCCTG23500.2521031842241337No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22710.24362822611617344No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22460.24094627734783158No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21830.23418776645161016No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21110.2264637539987856No Hit
TCTCTATACAGGAGACTCTGACTAGACCTACAGGAGATGGAGGCCGGCTC21000.2252836965407152No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20950.22474730678704682No Hit
GATTTTACACTGAAAATCAGCAGAGTGGGGCCTGAGGATGTTGGGGTTTATTACTGCATGCAAGTTCTACAAGGT20750.22260174777237338No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20440.21927613129962947No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20220.21691601638348865No Hit
CCTGTATAGAGATGGATACAACTATTTGAATTGGTACCTGCAGAAGCCAG18610.19964426631536714No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG18530.19878604270949776No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTC17970.192778477468412No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCC17420.18687819017805993No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT17410.18677091222732625No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17160.18408896345898443No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17030.18269435009944665No Hit
GAGTGGGGCCTGAGGATGTTGGGGTTTATTACTGCATGCAAGTTCTACAAGGTCCTTGGACGTTCGGCCAAGGGA16360.17550672740029052No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15990.17153744322314457No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGATGACTCAGTCTCCACTCTCCCTGTCCGTCACCC15960.17121560937094354No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGATGA15480.16606626773572722No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15440.16563715593279252No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCA15350.16467165437618944No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15250.16359887486885272No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15250.16359887486885272No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG15130.16231153946004861No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15020.16113148200197822No Hit
GTTTATTACTGCATGCAAGTTCTACAAGGTCCTTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAA14750.158234977332169No Hit
GTCTCCACTCTCCCTGTCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT14680.1574840316770333No Hit
CTCCTGTATAGAGATGGATACAACTATTTGAATTGGTACCTGCAGAAGCCAGGGCAGCCTCCACAGGTCCTGAT14310.15351474749988736No Hit
TTATTACTGCATGCAAGTTCTACAAGGTCCTTGGACGTTCGGCCAAGGGA14080.15104735463301286No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13990.15008185307640978No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13870.1487945176676057No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG13810.14815084996320366No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA13500.14482523349045978No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13150.14107050521478118No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13120.14074867136258015No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13100.14053411546111283No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGATGACTCAGTCTCCACTCTCC12990.1393540580030424No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12970.13913950210157505No Hit
CCACAGGTCCTGATCTATTTCGCTTCTAATCGGGCCTCTGGGGTCCCTGA12820.13753033284056992No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12760.1368866651361679No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12660.13581388562883115No Hit
GTCAGGGACCCCAGAGGCCCGATTAGAAGCGAAATAGATCAGGACCTGTGGAGGCTGCCCTGGCTTCTGCAGGTA12540.13452655022002707No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12480.13388288251562505No Hit
AGCCAGGGCAGCCTCCACAGGTCCTGATCTATTTCGCTTCTAATCGGGCC12450.13356104866342403No Hit
CAGTAATAAACCCCAACATCCTCAGGCCCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA12390.13291738095902197No Hit
ATACAGGAGACTCTGACTAGACCTACAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGACAGGGAGAGTGGAGA12150.1303427101414138No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12130.13012815423994645No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12090.12969904243701175No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGATGACTCAGTCTCCACT12070.1294844865355444No Hit
CTCCAGGGGTGACGGACAGGGAGAGTGGAGACTGAGTCATCACAATGTCC12020.12894809678187605No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12010.12884081883114235No Hit
GGATACAACTATTTGAATTGGTACCTGCAGAAGCCAGGGCAGCCTCCACAGGTCCTGATCTATTTCGCTTCTAAT11800.12658798186573522No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11770.1262661480135342No Hit
CTATACAGGAGACTCTGACTAGACCTACAGGAGATGGAGGCCGGCTCTCC11520.12358419924519234No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11500.123369643343725No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA11410.12240414178712193No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11340.12165319613198622No Hit
CCACTCTCCCTGTCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGTAG11300.12122408432905152No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11190.1200440268709811No Hit
GGTCTAGTCAGAGTCTCCTGTATAGAGATGGATACAACTATTTGAATTGGTACCTGCAGAAGCCAGGGCAGCCTC11060.11864941351144336No Hit
GCAGAAGCCAGGGCAGCCTCCACAGGTCCTGATCTATTTCGCTTCTAATCGGGCCTCTGGGGTCCCTGACAGGTT11050.11854213556070967No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10880.11671841039823722No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10790.11575290884163414No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10780.11564563089090046No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10710.11489468523576474No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10670.11446557343283005No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT10660.11435829548209638No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10570.11339279392549333No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10530.11296368212255863No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10360.11113995696008616No Hit
GGCACAGATTTTACACTGAAAATCAGCAGAGTGGGGCCTGAGGATGTTGGGGTTTATTACTGCATGCAAGTTCTA10350.11103267900935249No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10320.11071084515715146No Hit
CCTGTAGGTCTAGTCAGAGTCTCCTGTATAGAGATGGATACAACTATTTG10280.11028173335421677No Hit
CTCCCTGTCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGTAGGTCTA10220.10963806564981474No Hit
GGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC10200.10942350974834739No Hit
GATCTATTTCGCTTCTAATCGGGCCTCTGGGGTCCCTGACAGGTTCAGTG10050.10781434048734227No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10040.1077070625366086No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10030.10759978458587494No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC9850.1056687814726688No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9730.10438144606386471No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9710.10416689016239736No Hit
GTTCTACAAGGTCCTTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCAT9610.10309411065506063No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9550.10245044295065858No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)