FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences932158
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43490.46655180774074784No Hit
GTCCTTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC40760.43726492719045484No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29880.32054651679221763No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29510.3165772326150717No Hit
TCCAAGGACCTTGTAGAACTTGCATGCAGTAATAAACCCCAACATCCTCA26800.28750490796624606No Hit
GTATAGAGATGGATACAACTATTTGAATTGGTACCTGCAGAAGCCAGGGCAGCCTCCACAGGTCCTGATCTATTT26780.2872903520647787No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26030.2792445057597532No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25690.2755970554348083No Hit
GTAATAAACCCCAACATCCTCAGGCCCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA24170.2592908069232898No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22170.23783521677655503No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21000.2252836965407152No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20950.22474730678704682No Hit
TCTCTATACAGGAGACTCTGACTAGACCTACAGGAGATGGAGGCCGGCTC20350.21831062974302637No Hit
GATTTTACACTGAAAATCAGCAGAGTGGGGCCTGAGGATGTTGGGGTTTATTACTGCATGCAAGTTCTACAAGGT20210.21680873843275497No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20190.2165941825312876No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20160.2162723486790866No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20120.21584323687615187No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19730.2116593967975386No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19560.20983567163506614No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18730.2009316017241712No Hit
CCTGTATAGAGATGGATACAACTATTTGAATTGGTACCTGCAGAAGCCAG18660.20018065606903548No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGTAGGTCTAGTCAGAGTCTCCTG18350.1968550395962916No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17950.19256392156694466No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17830.19127658615814058No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17830.19127658615814058No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17530.18805824763613035No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTC17040.18280162805018035No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG16690.17904689977450175No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT16550.17754500846423033No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16430.17625767305542622No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15980.1714301652724109No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCC15790.1693918842084711No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCA15520.1664953795386619No Hit
GTTTATTACTGCATGCAAGTTCTACAAGGTCCTTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAA15480.16606626773572722No Hit
GAGTGGGGCCTGAGGATGTTGGGGTTTATTACTGCATGCAAGTTCTACAAGGTCCTTGGACGTTCGGCCAAGGGA15140.1624188174107823No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14660.15726947577556594No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA14650.15716219782483226No Hit
CTCCTGTATAGAGATGGATACAACTATTTGAATTGGTACCTGCAGAAGCCAGGGCAGCCTCCACAGGTCCTGAT14500.15555302856382716No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG14380.15426569315502306No Hit
GTCTCCACTCTCCCTGTCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT14060.1508327987315455No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13860.14868723971687203No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13500.14482523349045978No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG13460.14439612168752508No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13450.1442888437367914No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGATGA13410.1438597319338567No Hit
TTATTACTGCATGCAAGTTCTACAAGGTCCTTGGACGTTCGGCCAAGGGA13390.14364517603238935No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGATGACTCAGTCTCCACTCTCCCTGTCCGTCACCC13260.1422505626728516No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12870.13806672259423833No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12870.13806672259423833No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGATGACTCAGTCTCCACTCTCC12480.13388288251562505No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12380.1328101030082883No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12340.1323809912053536No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11610.12454970080179541No Hit
CCACAGGTCCTGATCTATTTCGCTTCTAATCGGGCCTCTGGGGTCCCTGA11570.1241205889988607No Hit
AGCCAGGGCAGCCTCCACAGGTCCTGATCTATTTCGCTTCTAATCGGGCC11550.12390603309739336No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11510.12347692129445866No Hit
CAGTAATAAACCCCAACATCCTCAGGCCCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA11440.12272597563932294No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11420.1225114197378556No Hit
GTCAGGGACCCCAGAGGCCCGATTAGAAGCGAAATAGATCAGGACCTGTGGAGGCTGCCCTGGCTTCTGCAGGTA11280.12100952842758415No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGACATTGTGATGACTCAGTCTCCACT11190.1200440268709811No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11160.11972219301878008No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11120.11929308121584538No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11020.11822030170850863No Hit
CTCCAGGGGTGACGGACAGGGAGAGTGGAGACTGAGTCATCACAATGTCC10830.11618202064456885No Hit
GGATACAACTATTTGAATTGGTACCTGCAGAAGCCAGGGCAGCCTCCACAGGTCCTGATCTATTTCGCTTCTAAT10820.11607474269383516No Hit
CTCCCTGTCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGTAGGTCTA10810.11596746474310149No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT10770.1155383529401668No Hit
GGTCTAGTCAGAGTCTCCTGTATAGAGATGGATACAACTATTTGAATTGGTACCTGCAGAAGCCAGGGCAGCCTC10760.11543107498943313No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10700.11478740728503108No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10620.11392918367916169No Hit
GGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG10590.11360734982696066No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10560.11328551597475964No Hit
ATACAGGAGACTCTGACTAGACCTACAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGACAGGGAGAGTGGAGA10490.11253457031962394No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA10340.11092540105861882No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10340.11092540105861882No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10300.11049628925568412No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT10040.1077070625366086No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10020.10749250663514125No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9970.10695611688147288No Hit
CTATACAGGAGACTCTGACTAGACCTACAGGAGATGGAGGCCGGCTCTCC9780.10491783581753307No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9710.10416689016239736No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9620.1032013886057943No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9380.10062671778818613No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9350.1003048839359851No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2700.052.989531
TGGTATC2800.049.8563462
GGTATCA8950.042.2478331
GACCGGA1003.6123765E-641.76598770
ACGCAGG909.5007184E-538.67220770
GTATCAA25450.035.6039621
TATCAAC25900.034.1796952
GCTTGTA502.0053072E-434.0646971
ATCAACG26700.033.651223
TCAACGC26900.033.6450354
CAACGCA27350.032.961755
AACGCAG27950.032.375886
TTTCGTA1107.6761353E-1032.316660
GCGCGAG553.3521108E-431.20929136
ACGCAGA29650.030.6343257
AGAGTAC29800.030.2518111
CGCAGAG30450.029.9412028
CAGAGTA30600.029.57208410
GTAGTTT950.005764189629.30946570
GCGCTTT1052.599154E-829.24071526
Produced by FastQC (version 0.11.5)