FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479833
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAGTGGTGCTCGAGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA47650.32199579276850837No Hit
CTCCTGGACCCCTGCCCGTTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTGCGCAGC47620.3217930671906898No Hit
GTCCAGGAGTGTCTGTTGGTTGTTTCATAAATCAGTGTCCTGGGGGCTTG39990.2702331952321647No Hit
CCACTATAGGTGAGCAAGCAGTAATAGTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTG36670.2477982312869087No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36580.247190054553453No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC35070.2369862004699179No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCG32660.22070057905182544No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA29170.19711683683226416No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28300.1912377950755254No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG28070.18968356564558297No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27500.18583177966703No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCGAGCACCACTATAGGTGAGCAAGCAGTAATAGTCAG27030.18265574561453893No Hit
GTAATAGTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAG27020.18258817042193276No Hit
GCCTGAGGATGAGGCTGACTATTACTGCTTGCTCACCTATAGTGGTGCTCGAGTGTTCGGCGGAGGGACCAAG26670.180223038680716No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT26640.1800203131028974No Hit
GACTATTACTGCTTGCTCACCTATAGTGGTGCTCGAGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG25530.17251946672361004No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG25160.1700191845971809No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAAACGGGCAGGGGTC24440.16515377072953502No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23660.159882905706252No Hit
CTATAGGTGAGCAAGCAGTAATAGTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCC23350.15778807473546No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23010.15549051818684945No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22180.14988177720053544No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21330.14413788582900908No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC21180.1431242579399162No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA21150.14292153236209762No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21070.1423809308212481No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT20960.14163760370257994No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG20820.14069155100609326No Hit
CTATTACTGCTTGCTCACCTATAGTGGTGCTCGAGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC20480.1383939944574827No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC20390.13778581772402695No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG20210.1365694642571155No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC20210.1365694642571155No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20110.13589371233105357No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19520.1319067759672882No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19370.1308931480781953No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC19340.13069042250037674No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18650.12602773421054944No Hit
CACCTATAGTGGTGCTCGAGTGTTCGGCGGAGGGACCAAGCTGACCGTCC18150.1226489745802398No Hit
GTCCAATTCCCAGGCTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAATCACTCTCAC17850.12062171880205402No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17810.12035141803162924No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAATCACTCTCACCTGTGGCTCCA16580.11203966934106753No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC16380.11068816548894368No Hit
CAGTAATAGTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGC16140.10906636086639505No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG16070.10859333451815172No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15800.1067688043177845No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15350.10372792065050584No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15220.10284944314662534No Hit
CCCTTTCGGGTGCGCAGCCTGAGGATGAGGCTGACTATTACTGCTTGCTC15160.10244399199098818No Hit
CCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAAACG15150.10237641679838197No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG15070.10183581525753244No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)