FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479833
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGACCCCTGCCCGTTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTGCGCAGC48110.3251042516283932No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45200.3054398705799911No Hit
CCTATAGTGGTGCTCGAGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA43760.29570904284469934No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG38440.25975904037820485No Hit
GTCCAGGAGTGTCTGTTGGTTGTTTCATAAATCAGTGTCCTGGGGGCTTG38260.25854268691129334No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC34220.2312423090983915No Hit
CCACTATAGGTGAGCAAGCAGTAATAGTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTG33270.2248226658008032No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCG28750.19427867874280408No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26960.1821827192662956No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26120.17650640308737542No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT25820.17447914730918962No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG25660.17339794422749052No Hit
GCCTGAGGATGAGGCTGACTATTACTGCTTGCTCACCTATAGTGGTGCTCGAGTGTTCGGCGGAGGGACCAAG25650.17333036903488436No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25310.17103281248627378No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG24520.16569437227038458No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA24330.1644104436108669No Hit
GTAATAGTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAG23760.16055865763231392No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCGAGCACCACTATAGGTGAGCAAGCAGTAATAGTCAG23620.1596126049358272No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA23460.15853140185412815No Hit
GACTATTACTGCTTGCTCACCTATAGTGGTGCTCGAGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG23420.15826110108370336No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAAACGGGCAGGGGTC21940.14825997257798684No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21810.14738149507410633No Hit
CTATAGGTGAGCAAGCAGTAATAGTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCC20970.14170517889518613No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20800.14055640062088087No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20390.13778581772402695No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT19990.13508281001977926No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19970.13494765963456687No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19870.13427190770850495No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19710.13319070462680585No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG19280.13028497134473957No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18600.12568985824751847No Hit
CTATTACTGCTTGCTCACCTATAGTGGTGCTCGAGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC18500.12501410632145654No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACA18210.12305442573587695No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC18100.12231109861720883No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17440.11785113590520012No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16920.1143372258896781No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16860.11393177473404094No Hit
CACCTATAGTGGTGCTCGAGTGTTCGGCGGAGGGACCAAGCTGACCGTCC16830.11372904915622237No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC16660.11258027088191709No Hit
GTCCAATTCCCAGGCTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAATCACTCTCAC16590.11210724453367374No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16520.11163421818543037No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC16480.11136391741500562No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC15960.10785000739948358No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15600.10541730046566065No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15440.10433609738396156No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG15360.10379549584311203No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15080.10190339045013863No Hit
CAGTAATAGTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGC14810.10007886024977143No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGT200.0078820450.859735
GGTATCA19650.042.326761
GTATCAA51850.040.2659451
TGGTATC6800.037.9339452
GTGGTAT7050.036.5961951
TATCAAC59900.034.7341772
CAACGCA62350.033.0092055
AACGCAG62800.032.9346476
ACGCAGA65100.031.8231437
CGCAGAG65300.031.6218268
AGAGTAC67000.031.0187611
CAGAGTA67450.030.66206410
GACAGGT3050.030.0521411
TCAACGC68750.029.9887054
ATCAACG69300.029.905583
GCAGAGT73200.028.7186099
GCTCGGG7550.028.34004870
GACCGGA1307.132075E-427.43171570
ATAAGGG500.00839546127.14078321
TAACGGC1653.6379788E-1226.98505836
Produced by FastQC (version 0.11.5)