FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1593670
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT48710.3056467148154888No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG45060.2827436043848476No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC41710.26172294138686175No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC40340.2531264314444019No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA36080.22639567790069462No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG33290.20888891677699903No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32350.20299058148801194No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC31210.19583728124392125No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT30230.18968795296391347No Hit
GATTATTACTGCAGCTCATATACAAGCTGCAATACTCCAATCTTCGGAACCGGGACCAAGGTCACCGTCCTGAGT28370.17801677888144973No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC28220.17707555516512202No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26780.1680398074883759No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC26440.16590636706469972No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT25730.16145124147408182No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25350.15906680805938495No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG25150.1578118431042813No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCACTGACATT25010.15693336763570878No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG24220.15197625606304943No Hit
GTCCTGAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC23170.14538769004875537No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG23040.14457196282793805No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG20510.12869665614587714No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTCAGGACGGTGACCTTGGTC19770.12405328581199371No Hit
CTCCTGCACTGGAACCAGCACTGACATTGGAGGTTATAATTCTGTCTCCTGGTACCAACAACACCCAGCCAAGGC19730.12380229282097296No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC19550.12267282436137972No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC19530.12254732786586935No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA19370.12154335590178644No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG19370.12154335590178644No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC19010.11928441898259991No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG18840.11821769877076183No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCACTGACATTGGAGGTTATAATTCTGTCTCCTGGTACCAACAACA18250.11451555215320612No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG18210.1142645591621854No Hit
GCCTTGGGCTGACTCAGGACGGTGACCTTGGTCCCGGTTCCGAAGATTGGAGTATTGCAGCTTGTATATGAGCTG18080.11344883194136803No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC17490.10974668532381232No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC17450.1094956923327916No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA17340.10880546160748461No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC17280.10842897212095352No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA17100.10729950366136025No Hit
ACTCAGGACGGTGACCTTGGTCCCGGTTCCGAAGATTGGAGTATTGCAGC16690.10472682550339782No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT16460.10328361580502865No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC16370.10271888157523201No Hit
GGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTGTCGGACATGACCAGGGA16080.10089918239033174No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT16020.10052269290380066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6100.046.9889871
TGGTATC6400.043.715922
GGTATCA20050.043.3982731
CTAGCGC3450.042.57550428
TAGCGCT3500.041.9817929
CGTATGC3000.041.44714441
CGCGTAT503.889356E-641.3289639
ACTAGCG3500.040.9681227
GACTAGC3700.038.74511326
GTATCAA50850.038.652551
AGGGGGG3550.037.06230570
CACGAGA4250.035.3128821
TACACAT4700.034.8424155
GAGACTA4350.034.5075924
TATACAC4700.034.118673
TCGTATG2850.033.8816640
TATCAAC58300.033.593022
ACGAGAC4500.033.35210422
CTTATAC4950.033.0889241
CGAGCCC4650.033.00770615
Produced by FastQC (version 0.11.5)