FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1251499
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44980.35940899673112003No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41160.3288856003880147No Hit
CTATTATATTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAATGAATTCTGTC40180.32105499085496675No Hit
GTACAAACTGGAGGCACCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTT38150.3048344425365102No Hit
ATATAATAGTTATTCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT33270.26584120322908766No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32630.2607273357789339No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31350.2504996008786264No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30230.24155033284085722No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28070.22429103019658828No Hit
CCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC27700.2213345755769681No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27240.21765898334717004No Hit
GATCTATGGTGCCTCCAGTTTGTACAGTGGGGTCCCATCAAGATTCAGCG27020.2159010914111797No Hit
CCTTCCACCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATCACTTGCCG25650.2049542189006943No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24600.19656428011528576No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG24060.1922494544542185No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23870.1907312750549541No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23760.1898523290869589No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23760.1898523290869589No Hit
GAATTCATTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA23660.18905328729787238No Hit
ATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAA23650.18897338311896372No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22510.17986430672337733No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22290.17810641478738695No Hit
GACTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGGTGCCTC22270.17794660642956966No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT22270.17794660642956966No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21990.17570928942012737No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA21630.17283273897941587No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT21220.1695566676441611No Hit
GTACAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAAT20790.16612078795108906No Hit
GTCTGCGTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA20480.16364375840492082No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC20310.16228538736347373No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC20260.16188586646893047No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC20040.1601279745329401No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19960.15948874110167088No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19640.15693180737659398No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT19240.15373564022024788No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18960.15149832321080559No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18730.1496605270959066No Hit
CAATATAATAGTTATTCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT17880.1428686718886711No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC17730.1416701092050413No Hit
CAGTTTGTACAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCATTCTCACCATCAGCAG17270.1379945169752433No Hit
ATTATATTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC17170.13719547518615677No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17080.13647633757597888No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16740.1337595954930847No Hit
CTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA16610.1327208411672722No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16450.13144237430473377No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15740.12576917760221942No Hit
GTCTCCTTCCACCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGTAG15730.12568927342331077No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15700.12544956088658482No Hit
CCACTGTACAAACTGGAGGCACCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAAC15640.12497013581313288No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAATGAATTCTGTCCCAGATCCACTGCCG14480.11570125105972917No Hit
ACTTATTACTGCCAACAATATAATAGTTATTCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA14460.11554144270191187No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14200.1134639340502869No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14180.11330412569246959No Hit
GCTACTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGACGCAGACAGGGTGGAAGGAGA14060.11234527554556575No Hit
GAATGAATTCTGTCCCAGATCCACTGCCGCTGAATCTTGATGGGACCCCA13870.11082709614630136No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13770.11002805435721484No Hit
CTCCTGTACAGGCTCTGGATTCACCTTTAGCAGCTATGCCATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG13770.11002805435721484No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13740.10978834182048887No Hit
GTATATACTGCCACCACTACCACTAATTCCTGCGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACT13610.10874958749467639No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13380.10691179137977737No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13210.10555342033833028No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC13160.105153899443787No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13090.10459457019142646No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13070.10443476183360913No Hit
ATCTTGATGGGACCCCACTGTACAAACTGGAGGCACCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG13020.10403524093906588No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12970.10363572004452261No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12950.10347591168670531No Hit
AGTTTGTACAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCATTCTCACCATCAGCAGC12880.10291658243434473No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12810.10235725318198416No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12690.10139840303508034No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12680.10131849885617167No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTA12680.10131849885617167No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12660.10115869049835437No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTCG350.004347698359.10880370
TACTCGA359.1823214E-442.62810566
GTGGTAT3450.042.1061131
TCGTATG2000.039.605340
TATGGCG459.839458E-538.44928743
CGTATGC2200.037.63004341
GTATCAA33100.037.4570771
CACGAGA3550.034.2558921
ATCTCGT2050.033.40883337
ACGAGAC3650.033.32402822
TATCAAC37750.032.7523572
CTTATAC4000.032.0936741
CCCACGA3800.031.9958219
CGGATGC553.2603616E-431.35836841
GTCGTAT553.3256577E-431.2525139
GACCCTA3900.031.23782226
TAAGACA4050.031.04197131
ATCTCCG3950.030.77832610
ATCAACG40400.030.6876183
TCAACGC41050.030.2839934
Produced by FastQC (version 0.11.5)