FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004686114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1251499
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43860.35045972869335096No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41830.3342391803748944No Hit
CTATTATATTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAATGAATTCTGTC39040.3119459144593803No Hit
GTACAAACTGGAGGCACCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTT35530.28389954766244324No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33460.26735938262835207No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32990.26360388621964537No Hit
ATATAATAGTTATTCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT32880.2627249402516502No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31130.248741708942636No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27430.21917716274643448No Hit
GATCTATGGTGCCTCCAGTTTGTACAGTGGGGTCCCATCAAGATTCAGCG25090.20047958488180972No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24910.199041309661454No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24400.1949661965371127No Hit
CCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC23930.19121070012840602No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23860.19065137087604545No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23370.18673606610952145No Hit
GAATTCATTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA22680.18122267776482442No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22220.1775470855350264No Hit
CCTTCCACCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATCACTTGCCG21200.16939685928634382No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21080.16843800913944No Hit
ATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAA20980.16763896735035344No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20650.1650021294463679No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA20190.1613265372165699No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20020.1599681661751228No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC19790.15813037006022376No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19790.15813037006022376No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19760.15789065752349782No Hit
GACTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGGTGCCTC19750.15781075334458916No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT19490.1557332446929642No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19390.1549342029038777No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19170.1531763109678873No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18790.15013995216935852No Hit
GTCTGCGTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA18540.14814234769664217No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18490.14774282680209894No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18320.14638445576065182No Hit
ATTATATTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC17810.14230934263631054No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17800.14222943845740188No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17740.14175001338394996No Hit
GTACAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAAT17250.13783470861742597No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17230.13767490025960868No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16800.1342390205665366No Hit
GAATGAATTCTGTCCCAGATCCACTGCCGCTGAATCTTGATGGGACCCCA16700.1334399787774501No Hit
CAATATAATAGTTATTCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT16650.1330404578829068No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC16080.1284859196851136No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16050.12824620714838766No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15340.1225730104458733No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT15270.12201368119351273No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15110.12073521433097431No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAATGAATTCTGTCCCAGATCCACTGCCG14920.11921703493170989No Hit
CTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA14860.11873760985825797No Hit
GTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCATT14660.11713952628008492No Hit
CAGTTTGTACAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCATTCTCACCATCAGCAG14390.1149821134495513No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14310.11434288001828208No Hit
CCACTGTACAAACTGGAGGCACCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAAC14150.11306441315574363No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14140.11298450897683499No Hit
GTCTCCTTCCACCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTAGTAG14030.11210556300883981No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG13900.11106680868302732No Hit
ACTTATTACTGCCAACAATATAATAGTTATTCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA13750.10986824599939754No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13550.10827016242122448No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13420.107231408095412No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13240.10579313287505623No Hit
GTATATACTGCCACCACTACCACTAATTCCTGCGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACT13080.10451466601251778No Hit
CTCCTGTACAGGCTCTGGATTCACCTTTAGCAGCTATGCCATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG13010.10395533676015721No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12860.10275677407652743No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12770.10203763646634956No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12660.10115869049835437No Hit
GCTACTAATAGTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGACGCAGACAGGGTGGAAGGAGA12640.10099888214053707No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGAATAA12550.10027974453035919No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12550.10027974453035919No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)