FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1450095
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC48060.33142656170802604No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGGATGTAACTGCCTGCATATGAG46340.31956526986162975No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG41960.2893603522527834No Hit
TCTCTGGGCTCCGGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATAT40700.2806712663653071No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGGATGTAA37760.2603967326278623No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37040.25543154069216156No Hit
GATTATTACTGCTGCTCATATGCAGGCAGTTACATCCATTGGGTGTTCGG35970.24805271378771734No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG34760.2397084328957758No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG34460.23763960292256717No Hit
GTCCTGGGCTCAGTTTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG33870.23357090397525682No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA31620.2180546791761919No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC31160.21488247321727197No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT30200.20826221730300426No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT29280.2019178053851644No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC29010.20005585840927662No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG28850.19895248242356536No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC28410.19591819846285935No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG27270.18805664456466645No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT25830.17812626069326493No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAAAAACACCCAGGCAATGC25520.17598846972094934No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC24750.17067847278971376No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24640.16991990179953728No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGGTCCTGGG24610.1697130188022164No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24550.16929925280757466No Hit
GTAATAATCAGCCTCATCCTCAGCCCGGAGCCCAGAGATGGTCAGGGAGG24260.16729938383347298No Hit
GTTACATCCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA23980.16536847585847822No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG23890.16474782686651565No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23850.16447198287008782No Hit
GGGCACAGGGTCCTGGGCTCAGTTTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC23740.1637134118799113No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC23670.16323068488616263No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23420.16150665990848875No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22740.1568173119692158No Hit
CCCCTGAGGGCCGCTTATTGACATCATAAATAATGAGTTTGGGGGCATTGCCTGGGTGTTTTTGGTACCAGGAGA22480.15502432599243499No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG22260.15350718401208197No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAAAAACA22100.15240380802637069No Hit
GGATGAGGCTGATTATTACTGCTGCTCATATGCAGGCAGTTACATCCATTGGGTGTTCGGCGGAGGGACCAAG21570.14874887507370207No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20590.14199069716122045No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20540.14164589216568568No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20340.14026667218354658No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC20050.1382668032094449No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGGATGTAAC19730.13606005123802234No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19680.13571524624248757No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19250.1327499232808885No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT18860.13006044431571726No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18430.12709512135411816No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18240.12578486237108602No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC18160.1252331743782304No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC17600.12137135842824091No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCGGG17590.12130239742913396No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT17110.1179922694720001No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16910.11661304948986102No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16750.11550967350414973No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16280.11226850654612285No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGGTCCTGGGCTCAGTTTGCCCTGACTCAGC16160.11144097455683938No Hit
CCCAATGGATGTAACTGCCTGCATATGAGCAGCAGTAATAATCAGCCTCA15980.11019967657291418No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG15880.10951006658184463No Hit
GGTTATAACTATGTCTCCTGGTACCAAAAACACCCAGGCAATGCCCCCAAACTCATTATTTATGATGTCAATAAG15790.10888941758988205No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15380.10606201662649688No Hit
CAGTTTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTC15370.10599305562738993No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15290.1054413676345343No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG15070.10392422565418129No Hit
GACCATCTCTGGGCTCCGGGCTGAGGATGAGGCTGATTATTACTGCTGCT15010.10351045965953955No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14960.10316565466400476No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14840.10233812267472131No Hit
CAGTTACATCCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT14690.10130370768811699No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14680.10123474668901004No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCTCAGTTTGCCCTGACTCAGCCT14680.10123474668901004No Hit
TCCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC14640.1009589026925822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4600.045.282571
GGTATCA16100.042.843461
TGGTATC5150.040.445172
GTATCAA39300.037.188641
ACCGATC2500.036.92133727
TATCTCG1600.034.39903636
ATCTCGT1450.033.24057837
ACGAGAC2900.032.9643322
GACCGAT2850.032.36813726
TATCAAC45200.032.3332562
GAGTTAC553.4617033E-431.0399826
AGACCGA3000.030.74018525
ATCAACG47450.030.7270453
TATACAC3450.030.6811563
TCAACGC48200.030.2478834
AACGCAG48900.029.9545366
CACGAGA3100.029.73625621
CAACGCA49150.029.7327025
CGTATGC1052.27883E-829.64142441
AATGGTA5600.029.54853670
Produced by FastQC (version 0.11.5)