FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1450095
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC46330.31949630886252284No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG42460.2928084022081312No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGGATGTAACTGCCTGCATATGAG38750.2672238715394509No Hit
TCTCTGGGCTCCGGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATAT37860.2610863426189319No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34720.23943258889934796No Hit
GATTATTACTGCTGCTCATATGCAGGCAGTTACATCCATTGGGTGTTCGG34330.23674310993417672No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG34230.23605349994310718No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33160.22867467303866298No Hit
GTCCTGGGCTCAGTTTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG32830.22639896006813345No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG31090.2143997462235233No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGGATGTAA30620.2111585792654964No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29610.20419351835569396No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG29470.20322806436819657No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT28620.19736637944410537No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27270.18805664456466645No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA27200.18757391757091776No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG25490.17578158672362845No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC25250.17412652274506152No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT25240.17405756174595458No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC24810.1710922387843555No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT24330.16778211082722166No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAAAAACACCCAGGCAATGC23990.1654374368575852No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGGTCCTGGG23990.1654374368575852No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23220.16012743992634965No Hit
GGGCACAGGGTCCTGGGCTCAGTTTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC23000.15861029794599665No Hit
GTTACATCCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA22700.15654146797278798No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22550.15550705298618367No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC21520.1484040700781673No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21360.147300694092456No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG21260.14661108410138646No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20980.1446801761263917No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20670.1425423851540761No Hit
GGATGAGGCTGATTATTACTGCTGCTCATATGCAGGCAGTTACATCCATTGGGTGTTCGGCGGAGGGACCAAG20580.1419217361621135No Hit
CCCCTGAGGGCCGCTTATTGACATCATAAATAATGAGTTTGGGGGCATTGCCTGGGTGTTTTTGGTACCAGGAGA20370.14047355518086746No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19980.13778407621569622No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAAAAACA19680.13571524624248757No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG19410.13385329926659978No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC19120.13185343029249808No Hit
GTAATAATCAGCCTCATCCTCAGCCCGGAGCCCAGAGATGGTCAGGGAGG19040.13130174229964245No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18640.12854330233536423No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18510.1276468093469738No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18470.127370965350546No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18030.12433668138983997No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC17820.12288850040859392No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGGTCCTGGGCTCAGTTTGCCCTGACTCAGC16780.1157165565014706No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT16320.11254435054255066No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGGATGTAAC16140.11130305255862548No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16060.11075136456576984No Hit
GGTTATAACTATGTCTCCTGGTACCAAAAACACCCAGGCAATGCCCCCAAACTCATTATTTATGATGTCAATAAG15740.10854461259434728No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC15560.10730331461042207No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCGGG15510.1069585096148873No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15480.10675162661756643No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15040.10371734265686042No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT15040.10371734265686042No Hit
TCCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC14800.1020622786782935No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14790.10199331767918654No Hit
GACCATCTCTGGGCTCCGGGCTGAGGATGAGGCTGATTATTACTGCTGCT14580.10054513669794049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41950.037.971341
GGTATCA17050.037.4100461
GTGGTAT5800.037.0496061
TATCAAC46900.033.518082
TGGTATC6400.033.0341222
ATCAACG49700.031.6863233
TCAACGC50050.031.4571424
CAACGCA50400.031.3052275
AACGCAG50700.031.1199876
CGCAGAG52550.030.1541188
AGAGTAC52850.029.72195611
ACGCAGA53450.029.5188677
CAGAGTA53750.029.2907110
AATGGTA5100.028.23277970
GCAGAGT61450.026.1749889
GTCTAGG801.0333515E-425.581871
CGGGTAC2550.025.39976117
TCCTATA6200.024.7498152
AGTACGG46950.024.68053813
GAGTACG47150.024.57500312
Produced by FastQC (version 0.11.5)