FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1411692
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTAACTGGGTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT51090.36190613816611555No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49920.3536182113378839No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47760.3383174233473024No Hit
GTTACTATACTGCTGACAGTAATAAACTGCTAGATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT43040.30488236810862424No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA37320.2643636147261584No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35850.2539505784547904No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34080.24141243274028615No Hit
GTTTATTACTGTCAGCAGTATAGTAACTGGGTCACTTTCGGCGGAGGGAC31800.2252616009724501No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31250.22136556699336682No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31160.22072803416042594No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29720.2105275088333716No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28680.20316046276383234No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28620.20273544087520506No Hit
GAGTATTAGCGCCAACTTAGCCTGGTTCCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAGC28230.19997279859912784No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATCTAGCAGTTTA27410.19416416612122192No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26760.18955976232775987No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26700.18913474043913261No Hit
GTAATAAACTGCTAGATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA26080.18474284758998422No Hit
GGACAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG26030.18438866268279483No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGAT25850.18311359701691304No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATC25020.17723412755756923No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24940.1766674317060662No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23890.16922954865508907No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT23850.16894620072933755No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCGCCAACTTAGCCTGGTT23640.1674586241191421No Hit
CATCTATGGTGCAGCCATCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA23180.16420012297299977No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23140.16391677504724828No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCGCCAACTTAGCCTGGTTCCAACAGAAACCTGGCCAGGCTCCCAGGCT22100.15654972897770902No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGGAACCAGGC21660.1534329017944424No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21260.15059942253692732No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC21030.14897017196385615No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20590.14585334478058953No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20100.14238233269013356No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCGC19480.13799043984098514No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19150.1356528194535352No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA19010.13466110171340492No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18730.13267766623314434No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18260.1293483281055641No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17850.12644401186661114No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17660.12509810921929146No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17400.12325634770190665No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17310.12261881486896577No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17040.12070621637014307No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT16940.1199978465557643No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT16750.11865194390844461No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16320.1156059537066159No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15910.11270163746766292No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15900.11263080048622505No Hit
GGATACCAGTGGCCCTGATGGCTGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGGAACCAGG15730.11142657180178113No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15610.11057652802452658No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15530.11000983217302358No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT15180.10753053782269788No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14940.1058304502681888No Hit
GCAGTATAGTAACTGGGTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT14690.10405952573224189No Hit
GTCTCTGGTGGCTCCATCATCAGTAGTACTTATTCCTACTGGGGCTGGAT14670.10391785176936612No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14590.1033511559178631No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14380.10186357930766768No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14370.10179274232622981No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14350.10165106836335404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14250.042.5514871
GTATCAA35600.038.275391
TCGTATG2550.033.81280540
CGTATGC2700.031.98249241
CTTATAC3100.031.8673481
TATCAAC43250.031.3455432
ATCAACG44000.030.9660743
TCAACGC44050.030.8525074
CAACGCA45000.030.2768675
AACGCAG45100.030.1331446
TGTTAGA16400.029.96966470
ACGCAGA47000.028.8415057
CGCAGAG47250.028.6889028
GTATGCC2900.028.61142742
ATGCCGT3050.028.41034344
TATACAC3500.028.2233663
AGAGTAC48450.028.11893711
GACCGGA1000.007101434728.08585570
GAGTTTA6750.027.7567441
GCAGAGT49000.027.7338079
Produced by FastQC (version 0.11.5)