FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1411692
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48220.3415759244934447No Hit
GTATAGTAACTGGGTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT45450.32195408063515274No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40760.2887315363407882No Hit
GTTACTATACTGCTGACAGTAATAAACTGCTAGATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT40030.2835604366958232No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35100.2486378048469496No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA34860.24693771729244057No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33160.2348954304480014No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33060.23418706063362263No Hit
GTTTATTACTGTCAGCAGTATAGTAACTGGGTCACTTTCGGCGGAGGGAC29070.20592310503990954No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27280.1932432853625295No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27210.19274742649246437No Hit
GAGTATTAGCGCCAACTTAGCCTGGTTCCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAGC26920.19069315403076592No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26440.1872929789217478No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25980.18403447777560544No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24980.1769507796318177No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATCTAGCAGTTTA24980.1769507796318177No Hit
GTAATAAACTGCTAGATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA24620.1744006483000541No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24100.1707171252652845No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATC24090.17064628828384662No Hit
GGACAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG24080.17057545130240875No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24070.17050461432097086No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGAT23300.1650501667502543No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23140.16391677504724828No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22660.16051659993823014No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT22550.1597373931424135No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22060.1562663810519575No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCGCCAACTTAGCCTGGTTCCAACAGAAACCTGGCCAGGCTCCCAGGCT21550.15265369499862577No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21420.15173281423993334No Hit
CATCTATGGTGCAGCCATCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA21280.15074109649980308No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCGCCAACTTAGCCTGGTT21150.14982021574111065No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20650.14627836666921679No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC20080.14224065872725777No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19420.13756541795235788No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18520.13119008962294892No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18460.13076506773432164No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA18360.13005669791994287No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGGAACCAGGC17950.1271523816809899No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCGC17540.12424806544203693No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17060.12084789033301882No Hit
GGATACCAGTGGCCCTGATGGCTGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGGAACCAGG16980.1202811944815158No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16720.11843943296413098No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16260.11518093181798862No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16110.11411837709642046No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15830.11213494161615989No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT15730.11142657180178113No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15380.10894727745145542No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15150.10731802687838424No Hit
GTCTCTGGTGGCTCCATCATCAGTAGTACTTATTCCTACTGGGGCTGGAT15080.1068221680083191No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14750.10448454762086914No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14430.10221776421485707No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14210.10065935062322376No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14170.10037600269747227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14950.043.2588921
GTATCAA36350.038.2048761
TATCAAC43750.031.8882182
AACGCAG44500.031.4836226
ATCAACG44450.031.3749223
TCAACGC44800.031.2739014
CAACGCA45500.030.7916725
ACGCAGA46950.029.9131367
CGCAGAG47450.029.741268
AGAGTAC47950.029.07345211
GCAGAGT48850.028.7496749
TGTTAGA18650.027.27356170
GTCGAAA500.00824865727.23902922
GACAGGT2900.024.648151
GCGTAGA32350.023.80971770
GAGTACG48900.022.81094212
AGTACGG48850.022.69666313
CAGGTAT1954.5474735E-1122.691951
GAGTTTA6800.022.5250951
CGGGTCA2750.022.27304817
Produced by FastQC (version 0.11.5)