FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2571191
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCCGCTCACTTTCGGCGGAGGGACCACGGTGGAGATCAAACGAACTGTGGCTGCACCAT56910.22133711575686132No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52220.2030965416415972No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48090.18703394652517064No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT46290.18003329974319293No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAATTCCATGTAGGCTGTGTCTGTAGATGTGTCCTCGGT46250.17987772981470457No Hit
GTGTATTACTGTGCAACCTTTTCCCCCAATTACTATGGTTCGGGGACTTA42760.16630425355409226No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42630.16579865128650498No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC41170.16012034889667862No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC38850.15109729304435182No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG36080.14032407549653061No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT35740.13900173110437927No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33370.12978421284144198No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA32610.1268283842001625No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT31350.12192793145277812No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT30740.11955549004333012No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA30410.11827203813330088No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29200.11356604779652697No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28890.11236038085074193No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT28730.11173810113678836No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGG27690.10769328299609013No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG27630.10745992810335754No Hit
GTGCAGCACCTCACCATGGACTGCACCTGGAGGATCCTCTTCTTGGTGGC27580.10726546569274707No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT27390.10652650853242719No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG27370.106448723568183No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27010.10504859421178744No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26750.10403738967661291No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCGTGGTCCCTCCGCCGAAAGTGAGCGGAGGCCAGTTATTATACTG26750.10403738967661291No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26340.10244279790960686No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG26160.1017427332314091No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG25980.10104266855321133No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25920.10080931366047874No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT25800.10034260387501356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26800.042.8420871
GTATCAA72450.037.2097241
TACGTAT2406.002665E-1132.359170
TATCAAC85150.031.6574842
ATCAACG86700.031.014083
TCAACGC87250.030.8179764
AACGCAG88400.030.6069366
CAACGCA88700.030.4277275
TAGCGCT2500.029.66644129
CTAGCGC2550.029.0495628
CGCAGAG94450.028.565328
ACGCAGA94750.028.51147
TCTAGCG606.253259E-428.05923328
CGTCTAA606.361777E-427.97681
AGAGTAC96850.027.96085711
ACTAGCG2650.027.92719527
GCGCTCA2850.026.07923931
TCGTATG1850.026.0065540
GACAGGT4550.025.824741
CAGAGTA105300.025.74895110
Produced by FastQC (version 0.11.5)