FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences777479
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC28900.3717142199339146No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT26620.34238866901871307No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA23590.3034165553024583No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23390.30084413855551084No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22600.2906830924050682No Hit
GATTATTACTGCTGCTCATATGCAAGTAGTGGCCCTTTGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTCGGT22150.2848951547244363No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC21610.277949629507678No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG19810.2547978787851505No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC18680.24026372416489702No Hit
GCCTTGGGCTGACCGAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCAAAGGGCCACTACTTGCATATGAGCAG17490.22495784452055942No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT17470.22470060284586466No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC16590.21338196915929564No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG16440.211452656599085No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16090.20695092729192685No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15680.20167747296068447No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG15640.20116298961129495No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC15300.1967898811414842No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCATTGGAACCAGCAGTGATGTT15180.1952464310933157No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCGAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCAAAGGGCCA15010.19305987685841033No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG14980.19267401434636822No Hit
GTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAA14950.19228815183432607No Hit
GTCGATCACCATCTCCTGCATTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGCA14750.18971573508737857No Hit
GACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT13440.17286640539487239No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG12980.16694984687689313No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG12610.16219087589504025No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC12520.1610332883589139No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA12360.15897535496135587No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG11320.14559878787722885No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC11100.1427691294555866No Hit
GCCCTGAGGGCCGCTTACTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA10290.1323508416304492No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC10170.1308073915822807No Hit
GGTTATAACTCCCAACATCACTGCTGGTTCCAATGCAGGAGATGGTGATC10000.1286208373473753No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC9870.12694876646185943No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA9810.12617704143777517No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA9710.12489083306430142No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9700.12476221222695402No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAAC9640.1239904872028698No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG9510.1223184163173539No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC9510.1223184163173539No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT9490.12206117464265917No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA9150.1176880661728484No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC8770.11280047435364815No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG8760.11267185351630075No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8390.10791288253444786No Hit
ACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA8340.10726977834771101No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG8300.1067552949983215No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC8210.10559770746219513No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG8150.10482598243811087No Hit
CCTCAGGGCTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCGACACGGCCTCCCTGACAATCTCTGGGCTCCAGG8100.10418287825137398No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT8070.10379701573933187No Hit
GACCGAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCAAAGGGCCACTACTTGCATATGAGCAGCAGTAATAAT8060.1036683949019845No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTCGCCAGACTTGGAGCC7910.10173908234177387No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7880.10135321982973174No Hit
GCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC7820.10058149480564747No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG7780.10006701145625799No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATT400.005859099354.81232570
TACGAAA450.00933404648.72206570
GTGGTAT3150.045.2244261
GGTATCA10100.043.321421
TGGTATC3550.041.0629922
GTATCAA24750.038.372241
ATAGCGG851.8371793E-938.36345763
CAAACGA951.9099389E-1036.7917449
ACGAACC805.9444574E-835.19757552
CTAGATT400.002409909435.02658550
GACCGGA850.002634148634.39204870
AATCGTT807.349081E-834.35893239
TAATCGT807.4252966E-834.31860738
TATCAAC28850.032.667022
TTAATCG851.2866076E-732.23681337
TCAACGC29450.031.9561964
TAGAATT1153.013166E-431.77526370
GGCGTGT450.003971582331.63151757
ATCAACG29850.031.4287133
ACAAACG1001.2985765E-831.40063948
Produced by FastQC (version 0.11.5)