FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700888
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC17710.25267945805891956No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT17670.25210875346703043No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC17370.2478284690278618No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT15980.227996484459714No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT13820.19717843649770006No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12550.17905856570521966No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12110.17278081519443905No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC11920.17006996838296562No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC11600.16550433164785244No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC11430.16307883713232357No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG11370.16222278024448986No Hit
GATTATTACTGCAGCTCATATGCAGGCAGCAACCATTTGAGGGTCTTCGG10520.1500953076668455No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9850.14053600575270228No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT9830.1402506534567577No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG9490.13539966442569995No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9400.1341155790939494No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC9390.1339729029459771No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9260.1321181130223374No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCGCCATCTCCTG9160.13069135154261452No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCTCAAATGGTTG8540.12184543036833276No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG8420.1201333165926653No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG8320.11870655511294244No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCTCAAATGGTTGC8160.11642373674538586No Hit
TCTCTGGGCTCCAGGCTGACGATGAGGCTGATTATTACTGCAGCTCATAT8100.11556767985755212No Hit
CTCCTGGACAGTCAGTCGCCATCTCCTGCACTGGAACCAGCAGTGACGTT8060.11499697526566299No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTTTCCTGGTACCAACAGCACCCAGGCAAAGC7900.1127141568981064No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT7540.1075778155711041No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC7050.10058668432046206No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)