FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1582308
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA46770.29558088564299745No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43400.27428288297853515No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36270.22922212363206151No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA34640.21892071581512576No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33490.2116528514044042No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31020.19604274262659355No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30050.18991245699320233No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT28460.17986384446011774No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26670.16855125550777725No Hit
GTATATTACTGTGCGAAAGATTTACGATTTTTGGAGTGGCTCAACCATGA25530.16134658991801848No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGGAGCCTGAAGATT25470.16096739699224172No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24390.1541419243282597No Hit
CTCCTGTGCAGCCTCTGGATTCACCTATAGCAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG24240.15319394201381778No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGTAGATACTTAGCCTGGTA24120.15243555616226423No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT23410.1479484398739057No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23050.14567328231924503No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23010.14542048703539387No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22780.14396691415324955No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT22520.14232374480821686No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22480.14207094952436566No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22450.14188135306147728No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA22080.13954299668585382No Hit
GTTTATTACTGTCAGCAGCGTAACAACCTAATGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA21330.1348030851136441No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21240.13423429572497894No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21230.13417109690401616No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20820.13157994524454153No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20710.13088475821395076No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA20690.13075836057202517No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGTAGATACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT20590.1301263723623972No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20390.12886239594314128No Hit
GGCCAAAACTGCACATTAGGTTGTTACGCTGCTGACAGTAATAAACTGCA20210.12772481716581097No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAACTG19790.12507046668537353No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19570.12368009262419201No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19140.12096254332279177No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19080.120583350397015No Hit
GTAATAAACTGCAAAATCTTCAGGCTCCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19010.12014095865027542No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18450.11660182467635882No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18350.11596983646673087No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18300.11565384236191689No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18070.11420026947977259No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17450.11028194258007923No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17280.10920756262371169No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17240.10895476733986051No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16650.10522603690305554No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16560.10465724751439037No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16490.1042148557676508No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC16470.10408845812572522No Hit
GACTTCACTCTCACCATCAGCAGCCTGGAGCCTGAAGATTTTGCAGTTTA16430.10383566284187402No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT15870.10029652886795744No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTA451.2375973E-463.97540370
TCTGGAG17650.045.26304670
GGTATCA13350.039.5603831
GTATCAA33800.038.8109321
TATCAAC38900.033.7140732
ATCAACG40950.032.2595373
GACAGGT4150.032.0203861
TCAACGC41650.031.7063334
AACGCAG41800.031.6709756
CAACGCA41750.031.6273885
GTTCATT16200.030.67896534
GCTTGTA1251.4551915E-1029.9842171
ATTTGCA16700.029.86214838
GCGTGTT17300.029.50690751
CGCAGAG44800.029.3982228
TTGCAGA17550.029.0715440
AGAGTAC45050.028.85645111
CGTGTTG17600.028.8493252
CGGCTCT17600.028.84587321
GCAGATA17500.028.62595442
Produced by FastQC (version 0.11.5)