FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214246
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36540.3009274891578807No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35610.2932684151316949No Hit
GTGTTAGCCTGTTGACAATAGTAAGTTGCAAAGTCTTCAGGCTGCAGGCTGCTGATGTTGAGAGTGAAATCTGTC32840.2704559043225178No Hit
GGCTAACACTTTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCAT30070.24764339351334078No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28060.23108991094061665No Hit
GGGTATTAACAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCATC25260.20803033322736908No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24840.20457139657038195No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24450.2013595268174653No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20910.17220563213714518No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20260.16685251588228414No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19010.15655806154601293No Hit
GTCTTCAGGCTGCAGGCTGCTGATGTTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGACGGGAC18380.1513696565605322No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18320.1508755227523912No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17980.1480754311729254No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17730.14601654030567118No Hit
GATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCGTCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA16730.1377809768366542No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16570.13646328668161148No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16550.13629857541223112No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAACAGCTGGTTAGCCTGGTATCAGCAGAAA16140.13292199438993416No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15550.12806301194321415No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15440.12715709996162228No Hit
CTCCTGATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCGTCAAGGTT15400.1268276774228616No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15350.12641589924941077No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC15350.12641589924941077No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14990.12345109640056462No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAACGTC14700.1210627829945497No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14300.11776855760694291No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14040.11562731110499849No Hit
GTTAATACCCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCTACAG14030.11554495547030832No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13930.11472139912340662No Hit
GCCTGAAGACTTTGCAACTTACTATTGTCAACAGGCTAACACTTTCCCGT13650.11241544135208187No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13040.1073917476359815No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12680.10442694478713539No Hit
GTGGGGTCCCGTCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT12610.1038504553443042No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCC12520.10310925463209268No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12400.10212098701581063No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12190.10039151868731708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT852.382876E-1065.6830170
GTACGTA951.4551915E-1146.7419469
GTGGTAT5050.042.9570661
GTATCAA38100.037.1875271
TATCAAC42650.032.5764242
TCAACGC44300.031.4240344
ATCAACG44100.031.4168223
AACGCAG44650.031.0234386
CAACGCA44800.030.920845
GGTACGT1151.63418E-830.67040368
TGGTACG1101.6778358E-830.5876867
AAGACCG450.005025830.14700723
GACCGGA500.006910285428.24490553
TTTCGAT1256.43826E-427.91527670
ACGCAGA51100.027.1738417
CGCAGAG53600.026.0959648
AGAGTAC54950.025.44960211
GCAGAGT55450.025.1631989
CAGAGTA55600.025.15518810
ATACGTA1951.8189894E-1224.310411
Produced by FastQC (version 0.11.5)