FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1247205
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGTTTAGTTTCAGGGAGA44740.3587221026214616No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35930.28808415617320327No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32150.257776388003576No Hit
CTATAAAAACTGGCCCATGTGCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCAT30690.2460702129962596No Hit
GTTTTTATAGTGCTGACAGTAATAAACTCCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAGT28460.22819023336179697No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA27930.2239407314755794No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25760.20654182752634895No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23430.18786005508316597No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22060.17687549360369786No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21070.16893774479736692No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGT19980.16019820318231565No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19970.16011802390144364No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCAGTAGCAAGTTAGCCTGGTTCCAGCAGAAACCTGGCCAGGCTCCCAGGCC19660.1576324661944107No Hit
GTATATTACTGTGCGAGAGGGGATATCTATTACTATGATAATAGTGGTCC19660.1576324661944107No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT19270.15450547424040154No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19190.1538640399934253No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18800.15073704803941615No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18250.1463271875914545No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18080.14496413981662998No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18040.14464342269314187No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGTAGCAAGTTAGCCTGGTT17800.14271911995221315No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGTAG16360.13117330350664086No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16310.1307724071022807No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16100.12908864220396807No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCA15990.12820667011437575No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC15750.12628236737344703No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCA15510.1243580646325183No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15370.12323555470030989No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15350.12307519613856584No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGGAGTTTATTACTGTCAGCACTATAAAAACTGG15300.12267429973420568No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14980.12010856274630073No Hit
CATCTATGATGCTTCTACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA14740.11818426000537201No Hit
GTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGCACATGGGCCAGTTTTTATAGTG14740.11818426000537201No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCC14680.11770318432013983No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14290.11457619236613066No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14160.11353386171479428No Hit
GGCCAAAAGTGCACATGGGCCAGTTTTTATAGTGCTGACAGTAATAAACTCCAAAATCTTCAGACTGCAGGCTG14060.11273206890607398No Hit
TCATAGATGAGGGGCCTGGGAGCCTGGCCAGGTTTCTGCTGGAACCAGGC13420.10760059493026407No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12840.10295019663968633No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12760.1023087623927101No Hit
CCAGTGGCCCTGGTAGAAGCATCATAGATGAGGGGCCTGGGAGCCTGGCCAGGTTTCTGCTGGAACCAGGCTAA12760.1023087623927101No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12520.10038445965178139No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12510.10030428037090937No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12510.10030428037090937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT10600.072.8927770
ACTACCG200.00740752351.6643240
GTCCGTA200.00782684450.94975312
CTACGGA11150.047.2021769
GGTATCA12800.042.4666441
TCTACGG12350.038.9958168
TTCTACG12100.037.8228867
GTATCAA30150.036.8466841
ACGTTTC12050.036.81260763
GACGTTT12050.036.693962
GGACGTT12150.036.2933461
TTGGACG12300.035.6471159
TTTCTAC13050.034.77880566
TCGTATG2300.034.44288640
TGGACGT12800.034.35872760
GTCATAT502.037597E-433.9733161
CTTATAC3300.033.9733161
CCGTTAA400.002800934233.9705916
CGTATGC2450.032.37361541
TATCAAC34400.032.195642
Produced by FastQC (version 0.11.5)