FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1247205
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGTTTAGTTTCAGGGAGA40060.32119819917335163No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37030.2969038770691266No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34070.27317080993100573No Hit
GTTTTTATAGTGCTGACAGTAATAAACTCCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAGT28370.2274686198339487No Hit
CTATAAAAACTGGCCCATGTGCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCAT28120.22546413781214797No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27470.220252484555466No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA26140.20958864019948606No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23820.19098704703717512No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22750.18240786398386793No Hit
GTATATTACTGTGCGAGAGGGGATATCTATTACTATGATAATAGTGGTCC19670.15771264547528274No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT19530.15659013554307433No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19010.15242081293772874No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18860.15121812372464832No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18290.14664790471494263No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18110.14520467765924608No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCAGTAGCAAGTTAGCCTGGTTCCAGCAGAAACCTGGCCAGGCTCCCAGGCC17950.1439218091652936No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGT17900.14352091276093346No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17420.139672307279076No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGGAGTTTATTACTGTCAGCACTATAAAAACTGG16380.13133366206838493No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16190.12981025573181634No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16110.1291688214848401No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16100.12908864220396807No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGTAGCAAGTTAGCCTGGTT16030.12852738723786386No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGTAG15910.12756523586739948No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14950.11986802490368464No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC14920.11962748706106856No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAGCGAACTGTGGCTGCACCA14770.1184247978479881No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14530.11650049510705938No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCC14320.11481673020874675No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCA14150.11345368243392226No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14030.1124915310634579No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13640.10936453910944871No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13640.10936453910944871No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13560.10872310486247248No Hit
GTGCAGCCACAGTTCGCTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGCACATGGGCCAGTTTTTATAGTG13330.1068789814024158No Hit
GGCCAAAAGTGCACATGGGCCAGTTTTTATAGTGCTGACAGTAATAAACTCCAAAATCTTCAGACTGCAGGCTG13220.10599700931282346No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13140.10535557506584722No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12910.10351145160579056No Hit
CATCTATGATGCTTCTACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA12560.10070517677526951No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT12480.10006374252829325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT10900.066.12363470
GTACGGT306.847413E-445.245621
CTACGGA10950.042.98706469
GGTATCA12350.041.2156451
GTATCAA31700.038.5372771
TCTACGG12150.034.6072968
TTCTACG11900.033.55919367
TATCAAC36700.033.2816242
GACGTTT11700.033.2213862
ACGTTTC11850.032.91575263
ATCAACG37750.032.25053
GGACGTT11950.032.1308261
AACGCAG38100.031.9465476
TCAACGC38100.031.8588394
TTGGACG12150.031.70616559
CAACGCA38400.031.6086755
TTTCTAC12450.031.5364866
GACAGGT2800.031.5103421
TGGACGT12150.031.22308360
CGTGTCT12400.030.08481613
Produced by FastQC (version 0.11.5)