FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297904
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT43961.4756431602126858No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC9280.3115097481067727No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG6740.2262473817068586No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC6440.21617702347064824No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT6350.21315591599978517No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA5840.1960363069982276No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT5760.19335087813523819No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGCCGT5620.18865137762500672No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT5570.18697298458563832No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT5390.18093076964391214No Hit
ATGCTGGAGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCC5360.1799237338202911No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG5250.17623126913368065No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG5220.17522423331005962No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC5170.17354584027069123No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG5000.1678393039368387No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG4600.15441215962189161No Hit
GATTACTACTGCGGAACATGGGATGACAGCCTGAATGCTGGAGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA4540.15239808797464954No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC4510.15139105215102852No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC4400.14769858746441808No Hit
CTCCAGCATTCAGGCTGTCATCCCATGTTCCGCAGTAGTAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGC4340.145684515817176No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT4280.14367044416993394No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTGTGAAAATAATAAGCGACCCTCAGGAATTCCTGACCGATTCTCTGG4240.1423277297384392No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCCAGCATTCAGGCTGTCATCCCATGTT4180.14031365809119717No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG4130.1386352650518288No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG4130.1386352650518288No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC4090.13729255062033408No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG4070.1366211934045867No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA4040.13561415758096568No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA4000.13427144314947095No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC3990.13393576454159728No Hit
GTAGTAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG3940.1322573715022289No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC3850.1292362640313658No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC3570.11983726301090283No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG3550.11916590579515549No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC3490.11715183414791341No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT3430.11513776250067136No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC3410.11446640528492401No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC3410.11446640528492401No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT3400.11413072667705033TruSeq Adapter, Index 12 (95% over 22bp)
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCCAGCATTCAGG3380.11345936946130297No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA3320.1114452978140609No Hit
GTTCCTGGGAGATGCTGGTACCACGATACAAAATTATTCGCAATGTTGGA3190.1070814759117031No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT3170.10641011869595575No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA3130.10506740426446104No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAACCACCTCCAACATTGCGAATAATTTTGTATCGTGGTACCAGCAT3040.10204629679359795No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)