FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047609
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51260.48930469287682715No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTGCGCAGC29790.28436181819743817No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29630.28283453082209103No Hit
CTACTGTAGGAGAGCAAGCAGTAATACTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTG29300.27968450061043765No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA29020.2770117477035803No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC28420.2712844200460286No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA24240.23138403736508564No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT24080.22985674998973854No Hit
GTATTACTGCTTGCTCTCCTACAGTAGTGTTCGGTTTTGGGTGTTCGGCG24020.2292840172239834No Hit
GTCCAGGAGTGTTTGTTGCTTGTATCATAAATCAATGTCCTGGGGGCTTG24010.22918856176302416No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTC23230.2217430358082071No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAACCGAACA23010.2196430156671048No Hit
CAGTAATACTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGC23010.2196430156671048No Hit
GTAATACTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAG22390.21372477708763482No Hit
GTAATATACAGCCGTGTCCTCAGGTCTCAGACTGCTCATTTGAAGATACA21980.20981110318830784No Hit
GAGTATTACTGCTTGCTCTCCTACAGTAGTGTTCGGTTTTGGGTGTTCGG20810.19864281425608218No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20740.19797462602936783No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG19880.18976545638687717No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT19680.18785634716769328No Hit
GTCCAATTCCCAGGCTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC19620.1872836144019381No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19440.18556541610467264No Hit
GCCTGAGGATGAGGCTGAGTATTACTGCTTGCTCTCCTACAGTAGTGTTCGGTTTTGGGTGTTCGGCGGAGGGAC19080.18212901951014168No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18780.17926535568136584No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA18380.1754471372429981No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18160.17334711710189585No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17900.17086527511695682No Hit
GTATATTACTGTGTCAAAGTGCCAGGATATTGTGTTGGTGGTAGGTGCTA17600.168001611288181No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTG17280.1649470365374868No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC16720.15960153072377192No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16100.15368329214430193No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCA16040.15311055937854676No Hit
GGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTCCAG15530.1482423308696279No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15330.14633322165044402No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15320.1462377661894848No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15320.1462377661894848No Hit
GCACTGGAGCTGTCACCAGTGGTCATTATCCCTACTGGTTCCAGCAGAAG15250.14556957796277045No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15150.14461502335317852No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15000.14318319143879063No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC14940.14261045867303546No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14740.14070134945385157No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG14720.14051043853193318No Hit
CCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTCCAGGAGTGTTTGTTG14500.13841041839083093No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCCCAGGC14410.13755131924219818No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14320.13669222009356544No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13740.1311558033579322No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC13350.12743304038052367No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12950.1236148219421559No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12670.12094206903529847No Hit
GTTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTT12600.12027388080858412No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC12070.11521474137774686No Hit
CACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCAGC11720.11187380024417508No Hit
CCTACAGTAGTGTTCGGTTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCC11650.11120561201746072No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11480.10958286918115441No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11410.10891468095444007No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11240.10729193811813377No Hit
AAGCTGCCCTGACCCTTTCGGGTGCGCAGCCTGAGGATGAGGCTGAGTAT11230.10719648265717459No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAACCGAA10650.10166006592154135No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10550.1007055113119494No Hit
TCCAATATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCAC10500.10022823400715343No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTGC200.007463715551.56510534
CTCGTAC200.00764970451.2450838
GTGGTAT3450.046.599271
CGAAGTA306.3402764E-445.95706638
TGGTATC3500.045.9203952
GGTATCA11600.041.2829171
GTATCAA29150.039.3103181
GACAGGT2250.038.0064961
TCTGGAG10700.035.14786570
GTCTTAA501.9573452E-434.2058451
TATCAAC33250.034.041772
ATCAACG34400.033.075693
TCAACGC34650.032.7353134
TTAACGG751.7092607E-632.10191735
AACGCAG35900.031.8794846
CAACGCA35750.031.8221235
TTACCGC450.004165480431.32606759
CGCAGAG37700.030.5386268
TGGTACC450.00484958630.3674586
AGAGTAC38050.029.9826411
Produced by FastQC (version 0.11.5)