FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836194
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATAGACTCCGTGCAGGGCCGGTTCACCATCTCCAGGGACAATTCCAGGAACACACTGTATCTACAAATGAA26620.31834717780802063No Hit
GTAATAAACGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGTAGATACA25670.30698617784868104No Hit
GTCTATATAGTATGTGCTATAACCACTTCCACTAATGCCTGAGACCCACT23220.27768675690091055No Hit
GGACTGAACAGATAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT18010.2153806413344272No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT16860.20162785190996346No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG16290.19481125193435972No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16250.1943328940413349No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAGAGTGACGCTGGTTGTATATGAGCTG16230.19409371509482248No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAGAGTGACGCTG15910.1902668519506239No Hit
CTCCTGTGCAGCCTCAAGATTCACCTTTAGCATCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG14960.1789058519912843No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14860.17770995725872224No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC14730.17615529410639158No Hit
GAGTCTATATAGTATGTGCTATAACCACTTCCACTAATGCCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGG13990.16730567308543232No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGGTAATTATTACTGCAGCTCATAT13970.1670664941389199No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG13890.16610977835287027No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATGACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC13330.15941276785052275No Hit
ATATAGACTCCGTGCAGGGCCGGTTCACCATCTCCAGGGACAATTCCAGG12840.15355288366096861No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC12820.1533137047144562No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATGACTATGTCTCCTGGTACCAACAACA12790.1529549362946876No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12540.14996519946328246No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12330.1474538205249021No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12030.14386613632721593No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11610.13884337845045527No Hit
GATCAGGACTGAACAGATAGAACTCACCATGGAGTTTGGGCTGAGCTGGC11300.13513610477951288No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT10950.13095047321554568No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC10950.13095047321554568No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10850.1297545784829836No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG10850.1297545784829836No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10530.12592771533878502No Hit
GGTTGTATATGAGCTGCAGTAATAATTACCCTCGTCCTCAGCCTGGAGCC10480.125329767972504No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG10430.12473182060622297No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC10380.12413387323994193No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG10350.1237751048201733No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT10340.1236555153469171No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10300.12317715745389227No Hit
GAGTAGGGTTCCCTGGCCCCAATAGTCAAGGGGATAACCACTAGGCTCAT10250.12257921008761125No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10190.12186167324807402No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT10090.12066577851551197No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGACGTGCAACTGTTGGAGT10070.12042659956899955No Hit
CCCTGAGGAGACGGTGAGTAGGGTTCCCTGGCCCCAATAGTCAAGGGGATAACCACTAGGCTCATAGTAAAAGGT9970.11923070483643748No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9910.11851316799690025No Hit
GTTTATTACTGTGCGAGGACCTTTTACTATGAGCCTAGTGGTTATCCCCTTGACTATTGGGGCCAGGGAACCCTA9480.11337082064688338No Hit
GGTTATGACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAT9370.11205533644106512No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9210.11014190486896581No Hit
ATATAGTATGTGCTATAACCACTTCCACTAATGCCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACC9050.10822847329686651No Hit
TATACAACCAGCGTCACTCTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC9040.10810888382361032No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8780.10499955751894896No Hit
TAGTAAAAGGTCCTCGCACAGTAATAAACGGCCGTGTCGTCGGCTCTCAG8640.10332530489336206No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8580.10260776805382484No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8470.10129228384800656No Hit
GAACAGATAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT8430.10081392595498173No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG8370.10009638911544451No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT150.00237424568.8607939
AGGGGGG2300.059.24806670
AGCCGGT350.004558853358.4016770
TCGCGTA309.340605E-657.27247238
TATCGCG601.8189894E-1257.0887336
GTATCGC601.8189894E-1256.96115535
ATCGCGT501.1768861E-954.8682937
AGTATCG1250.054.61014634
GGGTACG200.00794460850.756167
GTATCGG751.8189894E-1145.56892435
TTAACGG306.6100847E-445.56892435
GTGGTAT2850.045.127381
TGGTATC2850.045.124682
GGTATCA9900.043.7598841
ACGAGAC3900.042.52896522
CGATCAG3800.042.0047929
CACGAGA4050.040.9513721
ACCGATC3900.040.8664327
GACCGAT3900.040.8419526
AGACCGA4050.040.14684725
Produced by FastQC (version 0.11.5)