FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836194
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39150.46819278779804685No Hit
CTATATAGACTCCGTGCAGGGCCGGTTCACCATCTCCAGGGACAATTCCAGGAACACACTGTATCTACAAATGAA27620.33030612513364127No Hit
GTAATAAACGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGTAGATACA21280.25448639908920656No Hit
GTCTATATAGTATGTGCTATAACCACTTCCACTAATGCCTGAGACCCACT19620.23463454652867638No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18150.21705489396001407No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAGAGTGACGCTGGTTGTATATGAGCTG17620.21071665187743513No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAGAGTGACGCTG17420.20832486241231102No Hit
GGACTGAACAGATAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT17410.2082052729390548No Hit
CTCCTGTGCAGCCTCAAGATTCACCTTTAGCATCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG16400.196126736140178No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG16030.19170192562969837No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC15910.1902668519506239No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT15090.180460515143615No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG14670.17543775726685434No Hit
ATATAGACTCCGTGCAGGGCCGGTTCACCATCTCCAGGGACAATTCCAGG13810.16515306256682064No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGGTAATTATTACTGCAGCTCATAT13410.16036948363657239No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12830.15343329418771243No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC12650.15128068366910072No Hit
GAGTCTATATAGTATGTGCTATAACCACTTCCACTAATGCCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGG12590.15056314682956348No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATGACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC12510.14960643104351382No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12500.14948684157025763No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATGACTATGTCTCCTGGTACCAACAACA11390.13621241003881876No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA11040.13202677847485153No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC11040.13202677847485153No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC10870.12999375742949604No Hit
GGTTGTATATGAGCTGCAGTAATAATTACCCTCGTCCTCAGCCTGGAGCC10640.12724319954460328No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG10610.12688443112483466No Hit
GATCAGGACTGAACAGATAGAACTCACCATGGAGTTTGGGCTGAGCTGGC10320.12341633640040468No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10260.12269879956086746No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT10190.12186167324807402No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT10170.1216224943015616No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10030.11994824167597472No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT9920.11863275747015643No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9830.11755645221085059No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC9700.1160017890585199No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC9550.11420794695967681No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG9480.11337082064688338No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGACGTGCAACTGTTGGAGT9350.1118161574945527No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9280.11097903118175927No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9170.10966354697594098No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG8840.10571709435848618No Hit
TATACAACCAGCGTCACTCTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC8830.10559750488522998No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8730.10440161015266793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9900.041.012871
GCTTGCC751.0550139E-940.602741
GGATGGG7800.039.90107370
TGGTATC3100.038.1786422
GTGGTAT3250.037.4794541
GTATCAA23250.034.781561
CGCGCAA601.5376409E-533.8214421
CTAGCAC400.002869032333.803233
TATCAAC26000.030.824012
GCGCTTT450.00505339930.11217126
TCAACGC26950.029.7232194
ATCAACG27050.029.616883
CGCAAAT803.2195949E-629.59730323
AACGCAG27250.029.5164936
CAACGCA27350.029.408575
GCGCAAA704.4084074E-528.98980522
AGGATGG7300.027.93132669
ACGCAGA29100.027.6400157
TAGTGAC905.6230037E-627.55067353
CGCAGAG29450.027.5410358
Produced by FastQC (version 0.11.5)