FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1367809
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT65440.4784293713522868No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC57500.4203803308795307No Hit
GGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGAAAAGCCCCTAAGCTCCTGATCTATGGTGCATC49910.36489012720343267No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC45450.3322832354517334No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43830.32043947656434485No Hit
GATCTATGGTGCATCCAGGTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA43580.31861173599530346No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42840.31320162391094075No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAA38470.28125271876409647No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG38390.2806678417820032No Hit
CCATAGATCAGGAGCTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAGGC35920.26260976495987376No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC35470.2593198319355992No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG35320.2582231875941743No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG34680.25354417173742827No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33890.2477685115392573No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33380.24403992077841277No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31900.23321969660968747No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG31690.23168439453169265No Hit
ATTGTCAACAGGGTGACAGTTTCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAACTCAAACGAACTGTGGCTG31400.2295642154716046No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31120.22751714603427817No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29850.21823222394354766No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29770.21764734696145444No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29490.21560027752412803No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCCACTTACTATTGTCAACAGGGTGACAGTTTC29180.21333387921851663No Hit
GTGCAGCCACAGTTCGTTTGAGTTCCACCTTGGTCCCTTGGCCGAACGTC28780.21040949430805034No Hit
GTTGACAATAGTAAGTGGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCA28600.20909352109834048No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28550.2087279729845322No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT28080.2052918207147343No Hit
GGGTGACAGTTTCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAACTCAAACGAACTGTGGCTGCACCAT27250.19922372202551675No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGGGAAA26420.19315562333629915No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26150.1911816635217344No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26110.19088922503068775No Hit
GCTAATACCCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCTACAG24650.18021522010748578No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23900.17473199840036144No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC23810.17407401179550655No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23450.17144206537608686No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22690.16588573404620088No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22520.16464287045925272No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22420.16391177423163614No Hit
CTGTCACCCTGTTGACAATAGTAAGTGGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC22130.16179159517154806No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21650.15828233327898852No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21320.15586971572785382No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC21170.15477307138642896No Hit
GGACCAAGGTGGAACTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC20900.15279911157186418No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC20660.1510444806255844No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20400.14914363043378132No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19900.14548814929569845No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19700.1440259568404653No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT19560.1430024221218021No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19550.14292931249904045No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTTGCCACTTACTATTGTCAACAGGGTGACAGTTTCCCTCGGACGTT19250.14073602381619071No Hit
GTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAG19140.13993181796581247No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18680.13656877531877623No Hit
ACCTTGATGGGACCCCACTTTGTAACCTGGATGCACCATAGATCAGGAGCTTAGGGGCTTTTCCTGGTTTCTG18630.13620322720496794No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18530.13547213097735136No Hit
CCTTGGTCCCTTGGCCGAACGTCCGAGGGAAACTGTCACCCTGTTGACAATAGTAAGTGGCAAAATCTTCAGG18450.1348872539952581No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18410.13459481550421148No Hit
CTTTGTAACCTGGATGCACCATAGATCAGGAGCTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAGGCTAACCAG18330.13400993852211823No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTG18020.13174354021650683No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17810.13020823813851204No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17790.1300620188929887No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17710.12947714191089546No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17390.12713763398252242No Hit
GGTTAGCCTGGTATCAGCAGAAACCAGGAAAAGCCCCTAAGCTCCTGATC17360.12691830511423743No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC17260.12618720888662088No Hit
AGTCAGGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGAAA17220.12589477039557423No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA17070.12479812605414937No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17010.12435946831757944No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16990.12421324907205611No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16930.12377459133548617No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16630.12158130265263645No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16580.12121575453882816No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16490.12055776793397324No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15850.11587875207722716No Hit
AGGTGGAACTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG15740.11507454622684893No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15580.1139047922626624No Hit
GCCAAGGGACCAAGGTGGAACTCAAACGAACTGTGGCTGCACCATCTGTC15510.11339302490333081No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15440.11288125754399919No Hit
GGTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGC15240.11141906508876605No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCC15220.11127284584324273No Hit
GTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGAAAAGCCCCT15080.11024931112457953No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15050.11002998225629457No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG15040.10995687263353289No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15030.10988376301077124No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14680.10732492621411323No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14680.10732492621411323No Hit
GTCTCCTACAGATGCAGACACGGAAGATGGAGACTGGGTCATCTGGATGTCGCATCTGGAACCTGGGAACCAGAG14540.10630139149545002No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14410.10535096639954847No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14400.10527785677678682No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14360.10498541828574018No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG14060.10279212960289046No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC13980.10220725262079722No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATT13920.10176859488422725No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13700.10016018318347078No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT200.00741608951.649539
GTGGTAT2900.038.7096441
TACGTAT901.1402156E-437.4957170
GTATCAA34450.036.535561
GACAGGT2400.035.4349671
TATCAAC37750.033.251612
ATCAACG39900.031.37463
TCAACGC40200.031.3085564
CGTATGC1550.031.18270741
CAACGCA40950.030.817085
AACGCAG42000.030.0466546
TACGGGT2450.029.15365215
ATCTCGT1202.8267095E-928.61263337
ACGGGTA1203.1250238E-928.34486416
ACGCAGA44700.028.2296927
CGCAGAG45250.028.1120668
TGTTAGA18800.027.64311470
TAAGGCG2600.027.54622529
AATGGTA10300.027.52112470
TCGTCCG500.007918327.46812637
Produced by FastQC (version 0.11.5)