FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1367809
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT62810.45920154056597084No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44580.3259226982714692No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44390.3245336154389977No Hit
GGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGAAAAGCCCCTAAGCTCCTGATCTATGGTGCATC42090.30771840220381647No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41050.30011500143660413No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38370.28052162253647983No Hit
GATCTATGGTGCATCCAGGTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA37200.27196779667336596No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35500.2595391608038842No Hit
GGGTGACAGTTTCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAACTCAAACGAACTGTGGCTGCACCAT34150.2496693617310604No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC32150.2350474371787289No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31890.23314658698692584No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAA31020.22678604980666162No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30950.22627428244732997No Hit
CCATAGATCAGGAGCTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAGGC30820.22532385735142846No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30490.22291123980029376No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30130.22027929338087407No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29080.21260278299090005No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28990.2119447963860451No Hit
GTGCAGCCACAGTTCGTTTGAGTTCCACCTTGGTCCCTTGGCCGAACGTC28680.20967839808043376No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28420.20777754788863065No Hit
GTTGACAATAGTAAGTGGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCA27960.20441450524159444No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27640.20207499731322137No Hit
ATTGTCAACAGGGTGACAGTTTCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAACTCAAACGAACTGTGGCTG27630.20200188769045974No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27590.20170944919941308No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26740.1954951312646722No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26580.19432537730048569No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCCACTTACTATTGTCAACAGGGTGACAGTTTC26230.19176654050382766No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG26070.19059678653964113No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24650.18021522010748578No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT24130.17641351972387959No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23830.17422023104102985No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA23580.17239249047198843No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGGGAAA23500.17180761348989515No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC23410.17114962688504023No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23400.17107651726227857No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22940.16771347461524233No Hit
GCTAATACCCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCTACAG22450.1641311030999211No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22250.16266891064468797No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21800.15937897762041336No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG21760.15908653912936674No Hit
CTGTCACCCTGTTGACAATAGTAAGTGGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC21620.15806300441070356No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20640.1508982613800611No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20630.15082515175729944No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19960.1459268070322684No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19960.1459268070322684No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19170.14015114683409746No Hit
GGACCAAGGTGGAACTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC19110.1397124890975275No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18580.13583767909115965No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT18210.13313262304897833No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18140.13262085568964674No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18110.13240152682136175No Hit
CCTTGGTCCCTTGGCCGAACGTCCGAGGGAAACTGTCACCCTGTTGACAATAGTAAGTGGCAAAATCTTCAGG18080.13218219795307679No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17980.1314511017254602No Hit
CTTTGTAACCTGGATGCACCATAGATCAGGAGCTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAGGCTAACCAG17640.12896537455156384No Hit
GTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAG17420.1273569628508074No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC17220.12589477039557423No Hit
ACCTTGATGGGACCCCACTTTGTAACCTGGATGCACCATAGATCAGGAGCTTAGGGGCTTTTCCTGGTTTCTG17090.1249443452996727No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTTGCCACTTACTATTGTCAACAGGGTGACAGTTTCCCTCGGACGTT17050.12465190680862606No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16980.12414013944929446No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16870.12333593359891623No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16760.12253172774853797No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16660.12180063152092142No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16450.1202653294429266No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16420.12004600057464164No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16140.11799893113731523No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16010.11704850604141367No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15860.1159518616999888No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTGTAACCT15080.11024931112457953No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTG14940.10922577640591632No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14900.10893333791486969No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14790.10812913206449146No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT14750.10783669357344483No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14650.10710559734582825No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14610.10681315885478163No Hit
AGGTGGAACTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG14420.10542407602231015No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14400.10527785677678682No Hit
GGTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGC14340.10483919904021688No Hit
GTCTCCTACAGATGCAGACACGGAAGATGGAGACTGGGTCATCTGGATGTCGCATCTGGAACCTGGGAACCAGAG14300.10454676054917024No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG14210.10388877394431532No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCC13910.1016954852614656No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13720.10030640242899411No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT13710.10023329280623246No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13680.10001396393794748No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTC200.00688346252.63221452
GTGGTAT3550.046.9013561
GTATCAA39850.038.115081
TATCAAC42800.034.8401032
ATCAACG44700.033.343343
TCAACGC45150.032.923784
CAACGCA45950.032.3493845
AACGCAG46850.031.7267866
GACAGGT2750.030.8905051
ACGCAGA49450.030.0586437
CGCAGAG49850.029.8855278
AGAGTAC49800.029.8430211
GCAGAGT51150.029.2565279
CAGAGTA51200.029.16167810
CAGGTAT1750.027.1836431
GTCTACT500.00833087627.1836431
AGCGTAG1307.9022517E-426.95419370
AATGGTA10650.026.32146670
TCGCTAT659.404865E-426.1649425
CTCGCTA659.441137E-426.1476924
Produced by FastQC (version 0.11.5)