FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1881531
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCTTCGGCTCTCAGGTTGTCCATTTGCAGATAGA66750.3547642850423405No Hit
GTAATATACTGCAGAGTCTTCAGGCTCCAGTCTAGTGATGCTGAGAGTGAAGTCTGTCCCAGACCTACTGCCA61400.3263299940314563No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCACGCGACCCCGAGAGCCT59220.3147436847971147No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT54290.2885416185011036No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT53790.28588420812625465No Hit
GTATATTACTGTCTGCAGTATAGTCACTCACGGTACACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAA51360.2729691937044885No Hit
GTGTATTACTGTGCGAGAGATCCCGGTGCAGGTGGGAGTTACGGGGACAA49810.26473122154245665No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT42980.22843099582201942No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42630.2265708085596251No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTGATAATTGGATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG37670.2002092976411231No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG36580.1944161430239523No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC36080.19175873264910331No Hit
CAGTAATATACTGCAGAGTCTTCAGGCTCCAGTCTAGTGATGCTGAGAGTGAAGTCTGTCCCAGACCTACTGCCA35650.18947335972673318No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGGCAG34980.1859124298244355No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA34230.18192631426216205No Hit
ATATTACTGTCTGCAGTATAGTCACTCACGGTACACTTTTGGCCAGGGGA34090.18118223935720432No Hit
CAGTAATACACGGCCGTGTCTTCGGCTCTCAGGTTGTCCATTTGCAGATA33480.17794019869988856No Hit
GGGTACGTGCCAAGCATCCTCACGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCCGGCCGTGGCACTCATTTA33210.1765051970974701No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33070.1757611221925124No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACTCATCCAATTATCAC32550.1729974154026694No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT31620.1680546321054503No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC31380.16677907512552279No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG31080.1651846289006134No Hit
GTATTACTGTGCGAGAGATCCCGGTGCAGGTGGGAGTTACGGGGACAACTGGTTCGACCCCTGGGGCCAGGGAAC30580.1625272185257644No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGACAGTTGAGCGCAAATGTTGTGT29180.1550864694761872No Hit
CAACTGTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATCTACGTTGCAGGTGTAGGTCTGGGTGCCCAAGCTG29090.1546081356087144No Hit
GTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA28910.15365146787376874No Hit
CCGCTGGTCAGAGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA27450.1458918295792097No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC27180.14445682797679124No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCAGCTACTTAGCCTGGTACC26680.14179941760194226No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTTGAGT26640.14158682477195433No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26540.14105534269698453No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26130.13887626618960836No Hit
GGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTCCCCGTAACTCCCACCT25720.13669718968223218No Hit
CTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACC25380.13489015062733486No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG24840.13202014742249796No Hit
CCTTCCCAGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG24840.13202014742249796No Hit
ACATAAAGCAAGATGGAAATTATCAAGACTATGCGGACTCTGTGAAGGGCCGATTCACCGTCTCCAGAGACAACG24700.13127607251754025No Hit
GTCCCAGACCTACTGCCACTGAACCTGTCTGGGATGCCAATGGCCCTGCT24490.13015996016010367No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG24310.12920329242515802No Hit
GGGCTGGAGTGGGTGGCCAACATAAAGCAAGATGGAAATTATCAAGACTA24060.12787458723773354No Hit
GCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC23770.1263332892203211No Hit
GTGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT23440.12457939837292077No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23120.12287865573301741No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC23010.12229402545055064No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAG22810.12123106130061104No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAA22520.11968976328319862No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21870.11623512979589494No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21860.11618198158839796No Hit
GCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAG21750.11559735130593118No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGG21650.11506586923096139No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT21440.11394975687352481No Hit
GCCACAGGTGGTGCTGGGCACGGTGGGCACTCGACACAACATTTGCGCTCAACTGTCTTGTCCACCTTGGTGTTG21260.11299308913857917No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTT21230.11283364451608822No Hit
GTATAGTCACTCACGGTACACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCAT21060.11193012498863956No Hit
GAGGAAGACTGACGGTCCTGCCACAGGTGGTGCTGGGCACGGTGGGCACTCGACACAACATTTGCGCTCAA21060.11193012498863956No Hit
GTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGA21050.11187697678114258No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCTCTGACCAGCGGCGTGCACACCTTC20870.11092030904619696No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGGCTCACGTCCACCACCACGCACGTGA20580.10937901102878453No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA20540.10916641819879662No Hit
GTTCAACAGCACGTTCCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG20540.10916641819879662No Hit
GTCTCAGAGAGGAGCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCA20530.10911326999129964No Hit
GTCTGGGACAGACTTCACTCTCAGCATCACTAGACTGGAGCCTGAAGACT20440.10863493612382681No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20400.1084223432938389No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20360.10820975046385098No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20330.10805030584136004No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGTGATAATTGG20020.10640271140895366No Hit
GTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAAC19980.10619011857896574No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG19930.10592437754148085No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGG19830.10539289546651104No Hit
CTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCA19220.10215085480919528No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGC19150.10177881735671643No Hit
GTGTGAGGTGCAGCTGGTTGAGTCTGGGGGAGGCTTGGTCCAGCCGGGGGGGTCCCTGAGACTCTCCTGTGCAGC18950.10071585320677683No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT18910.1005032603767889No Hit
GTGCTGGGCACGGTGGGCACTCGACACAACATTTGCGCTCAACTGTCTTG18820.10002492650931609No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAG200.007573859351.3760074
ATACCGT200.007573859351.3760076
GGTATCA10050.046.351631
CTTATAC2950.040.638551
CGCAATT350.001206659140.327160
CGTTGTA753.130117E-837.8636463
GTATCAA23800.037.850441
TTATACA3100.037.566252
TCGTATG655.450074E-737.1569640
TCTCCGA3000.036.5253111
TATACAC3350.035.7852333
CCTTTGT22050.035.54020770
ATCTCCG3200.035.3116210
GTTTGCG400.002367830835.15527358
CGAGACT3350.034.7609523
ACGAGAC3500.033.2676622
CACGAGA3500.033.26677721
TTAACGG1450.033.20820235
CGTATGC958.712959E-932.71849441
ATACACA3800.032.4480064
Produced by FastQC (version 0.11.5)