FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1881531
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACTGCAGAGTCTTCAGGCTCCAGTCTAGTGATGCTGAGAGTGAAGTCTGTCCCAGACCTACTGCCA59780.31771998441694554No Hit
GTAATACACGGCCGTGTCTTCGGCTCTCAGGTTGTCCATTTGCAGATAGA59410.3157535007395573No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT53040.28189809256398113No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCACGCGACCCCGAGAGCCT52000.27637067898429524No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT47500.2524539856106543No Hit
GTGTATTACTGTGCGAGAGATCCCGGTGCAGGTGGGAGTTACGGGGACAA46720.24830842542588988No Hit
GTATATTACTGTCTGCAGTATAGTCACTCACGGTACACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAA46550.24740490589844122No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT46280.24596990429602275No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41220.21907691130255094No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37270.1980833693412439No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTGATAATTGGATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG37240.19792392471875297No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC35600.18920761868924826No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG32850.1745918616275788No Hit
CAGTAATATACTGCAGAGTCTTCAGGCTCCAGTCTAGTGATGCTGAGAGTGAAGTCTGTCCCAGACCTACTGCCA31070.16513148069311642No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGGCAG30620.16273981135575233No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA30200.16050758664087916No Hit
GGGTACGTGCCAAGCATCCTCACGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCCGGCCGTGGCACTCATTTA29920.15901943683096373No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACTCATCCAATTATCAC29830.1585411029634909No Hit
ATATTACTGTCTGCAGTATAGTCACTCACGGTACACTTTTGGCCAGGGGA28860.15338572683628385No Hit
GTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA28750.15280109655381707No Hit
GTATTACTGTGCGAGAGATCCCGGTGCAGGTGGGAGTTACGGGGACAACTGGTTCGACCCCTGGGGCCAGGGAAC28390.15088776108392582No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAA27900.1482834989165738No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTT27800.147752016841604No Hit
CAGTAATACACGGCCGTGTCTTCGGCTCTCAGGTTGTCCATTTGCAGATA27750.1474862758041191No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG27550.1464233116541795No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC27100.1440316423168154No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT26460.14063015703700868No Hit
GGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTCCCCGTAACTCCCACCT26410.1403644159995238No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26160.13903571081209928No Hit
GGGCTGGAGTGGGTGGCCAACATAAAGCAAGATGGAAATTATCAAGACTA26150.13898256260460232No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC25800.137122375342208No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGACAGTTGAGCGCAAATGTTGTGT25510.13558107732479563No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25220.1340397793073832No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTTGAGT24840.13202014742249796No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA24750.13154181355502514No Hit
GTCCCAGACCTACTGCCACTGAACCTGTCTGGGATGCCAATGGCCCTGCT24350.12941588525514594No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24260.12893755138767313No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23880.12691791950278788No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCAGCTACTTAGCCTGGTACC23080.12266606290302949No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22800.12117791309311406No Hit
CAACTGTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATCTACGTTGCAGGTGTAGGTCTGGGTGCCCAAGCTG22770.12101846847062311No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22610.12016809715067144No Hit
CTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACC22510.11963661507570164No Hit
CCGCTGGTCAGAGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA22350.11878624375574998No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG22330.11867994734075601No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT22060.11724494573833756No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG21920.11650087083337984No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA21910.11644772262588286No Hit
CCTTCCCAGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG21210.11272734810109428No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC21140.11235531064861541No Hit
GTCTGGGACAGACTTCACTCTCAGCATCACTAGACTGGAGCCTGAAGACT20980.11150493932866373No Hit
GCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAG20880.11097345725369394No Hit
GTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGA20240.10757197197388722No Hit
GTGTGAGGTGCAGCTGGTTGAGTCTGGGGGAGGCTTGGTCCAGCCGGGGGGGTCCCTGAGACTCTCCTGTGCAGC20220.10746567555889326No Hit
ACATAAAGCAAGATGGAAATTATCAAGACTATGCGGACTCTGTGAAGGGCCGATTCACCGTCTCCAGAGACAACG20200.1073593791438993No Hit
GAGGAAGACTGACGGTCCTGCCACAGGTGGTGCTGGGCACGGTGGGCACTCGACACAACATTTGCGCTCAA20060.10661530423894158No Hit
GCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC19790.10518030263652312No Hit
GTCTCAGAGAGGAGCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCA19710.10475511697654728No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAG19600.10417048669408051No Hit
GTGCTGGGCACGGTGGGCACTCGACACAACATTTGCGCTCAACTGTCTTG19520.10374530103410466No Hit
GTATAGTCACTCACGGTACACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCAT19270.10241659584668018No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCTCTGACCAGCGGCGTGCACACCTTC19200.10204455839420133No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19000.10098159424426173No Hit
GTGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT18930.10060955679178286No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGGCTCACGTCCACCACCACGCACGTGA18820.10002492650931609No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT204.3889624E-4105.3356370
CGTCTAA354.026235E-580.2557270
CGTATAA350.00404672960.19178870
ACGCTTG305.8495847E-446.7163255
GGTATCA10450.043.8665581
GTATCAA24800.036.1405451
TCTGGAG30400.034.6498870
CGTTGTA1107.748895E-1032.29205363
GACAGGC2700.031.6753431
TATCAAC28650.030.7924142
ATCAACG28700.030.7256953
CCTTTGT27850.030.51014170
ATTGGCG1051.934859E-830.1452958
TCAACGC29450.030.0536734
AACGCAG29950.029.666046
CAACGCA30350.029.2750575
CTCGTAT853.6974361E-629.0792660
TATAGGG1256.210882E-428.08950270
GTTCGGT500.00717413228.02979555
AGAGTAC32550.027.81837811
Produced by FastQC (version 0.11.5)