FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences926683
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22550.24334103463643986No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21750.23470809327461495No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21030.2269384460489725No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20150.2174422105509651No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT18360.19812600425388188No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17980.19402535710701502No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT17950.19370162180594658No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17180.1853924157451901No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17090.1844212098419848No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC16730.18053638622916357No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16500.17805441558763893No Hit
CCCCAGGCCAGCCTCCACGCACGCTCATCTACGGCACAAACATTCGCTCC16250.17535662141206862No Hit
CTACTAGTCACTACCCCAGCTGGTATCAGCAGACCCCAGGCCAGCCTCCA16000.17265882723649834No Hit
GATTACTACTGTTTACTTTATATGGGTGGTGGCATGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTACGTCAG15950.17211926840138428No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCAT15930.1719034448673387No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCCTGGTCCCTC15800.1705005918960421No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15420.16639994474917527No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15370.16586038591406121No Hit
GTAGTAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAG14880.16057270932994347No Hit
CCCCAGAGGAGCGAATGTTTGTGCCGTAGATGAGCGTGCGTGGAGGCTGG14830.1600331504948294No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGGCAT14820.1599252387278066No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14510.15657997395009943No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14460.15604041511498537No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG14300.15431382684262038No Hit
GTGGTGACCCAGGAGGCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGACCTCTGG14230.15355844447346073No Hit
GTCTCTACTAGTCACTACCCCAGCTGGTATCAGCAGACCCCAGGCCAGCC14170.15291097387132385No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14040.1515081209000273No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG13790.14881032672445701No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12950.13974573829454084No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC12690.13694003235194774No Hit
GACGTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCATGCCACCACCC12660.1366162970508793No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12540.13532135584660557No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC12390.1337026793412634No Hit
CCTCTGGGGTCCCTGACCGCTTCTCTGGCTCCGTCCTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGG12380.1335947675742406No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12240.13208400283592123No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12140.1310048851656931No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12090.13046532633057906No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11860.12798335568905442No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11720.12647259095073504No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT11650.12571720858157537No Hit
GTGTTCGGCGGAGGGACCAGGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACC11610.1252855615134841No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11610.1252855615134841No Hit
CCCATATAAAGTAAACAGTAGTAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTC11040.11913459079318385No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC10990.11859503195806981No Hit
CTCATCTACGGCACAAACATTCGCTCCTCTGGGGTCCCTGACCGCTTCTCTGGCTCCGTCCTTGGGAACAAAG10770.11622097308356795No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10560.1139548259760889No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10310.11125703180051862No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10140.10942253176113083No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT9980.10769594348876584No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC9920.10704847288662898No Hit
CACTTGTGGCTTGACCTCTGGCTCAGTCTCTACTAGTCACTACCCCAGCTGGTATCAGCAGACCCCAGGCCAGC9780.10553770814830962No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT9630.10391903164296745No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9610.10370320810892182No Hit
CACCATCACGGGGGCCCAGGCAGATGATGAATCTGATTACTACTGTTTACTTTATATGGGTGGTGGCATGGTGTT9580.10337947280785338No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG9300.10035794333121467No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCCTGGTCCCTCCGCCGAACAC9300.10035794333121467No Hit
AGTAGAGACTGAGCCAGAGGTCAAGCCACAAGTGAGTGTGACTGTCCCTCCAGGGGACACTGAGAACGATGCCTC9300.10035794333121467No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATCG200.007560042751.39764434
AGGGGGG2500.044.27286570
CGTATGC2600.042.51878741
ATCTCGT2400.041.53448537
GTGGTAT3400.041.0676571
TCTCGTA2600.041.02609638
AATGGTA1706.366463E-1140.6919770
TATCTCG2450.040.64698436
GTATGCC3000.040.3617242
TCGTATG2650.039.06434240
GCCTATC3000.038.80006433
CACGAGA3250.038.7652921
GTATCAA39100.038.759511
GCCGTCT2650.038.0724446
GAGCCTA3350.037.75481831
ATGCCGT3050.037.53025444
TGCCGTC3000.037.0565745
TATACAC3450.036.5239373
CTTATAC3700.034.976541
CTATCTC2950.034.89703435
Produced by FastQC (version 0.11.5)