FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences926683
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35580.38395006706716317No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22410.2418302698981205No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20480.2210032988627179No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19370.2090250927231858No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA18650.20125544549754337No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18430.19888138662304153No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17890.1930541512038097No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT17540.18927723935801133No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT17490.18873768052289724No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC15570.16801862125451747No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15420.16639994474917527No Hit
CTACTAGTCACTACCCCAGCTGGTATCAGCAGACCCCAGGCCAGCCTCCA15400.16618412121512965No Hit
GTGGTGACCCAGGAGGCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGACCTCTGG15080.1627309446703997No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15040.16229929760230846No Hit
GATTACTACTGTTTACTTTATATGGGTGGTGGCATGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTACGTCAG14450.15593250334796255No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG14380.1551771209788029No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGGCAT14370.15506920921178008No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14140.1525872385702554No Hit
CCCCAGGCCAGCCTCCACGCACGCTCATCTACGGCACAAACATTCGCTCC13980.15086065029789045No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCAT13910.15010526792873075No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13300.14352265014033924No Hit
GTAGTAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAG13160.1420118854020199No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCCTGGTCCCTC13110.14147232656690584No Hit
CCTCTGGGGTCCCTGACCGCTTCTCTGGCTCCGTCCTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGG13090.14125650303286022No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13070.1410406794988146No Hit
CCCCAGAGGAGCGAATGTTTGTGCCGTAGATGAGCGTGCGTGGAGGCTGG12960.13985365006156367No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12450.13435014994340028No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG12410.13391850287530904No Hit
GTCTCTACTAGTCACTACCCCAGCTGGTATCAGCAGACCCCAGGCCAGCC12240.13208400283592123No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCCTGGTCCCTCCGCCGAACAC12180.13143653223378438No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11740.1266884144847807No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC11330.12226403203684538No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11240.12129282613364009No Hit
GACGTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCATGCCACCACCC11140.12021370846341198No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11070.11945832609425229No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11000.11870294372509263No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10940.11805547312295575No Hit
GTGTTCGGCGGAGGGACCAGGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACC10920.11783964958891012No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC10910.11773173782188731No Hit
CTCATCTACGGCACAAACATTCGCTCCTCTGGGGTCCCTGACCGCTTCTCTGGCTCCGTCCTTGGGAACAAAG10820.11676053191868202No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10760.11611306131654514No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10140.10942253176113083No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGG10070.10866714939197115No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC9760.10532188461426399No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9720.10489023754617274No Hit
GTATCAACGCAGAGTACGGGAGCTGGGAAGCCTGAGAACACTTAGCCTTC9680.1044585904780815No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9630.10391903164296745No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9570.10327156104083057No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT9310.10046585509823748No Hit
GGCCTGGGGTCTGCTGATACCAGCTGGGGTAGTGACTAGTAGAGACTGAG9290.10025003156419185No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGAG200.0076279351.28154832
GTATCAA39600.040.4254531
GGTACCT952.582965E-1035.77327319
CGGGTAC1151.8189894E-1235.4583717
GCGATTT800.002286342235.40126870
GTGGTAT3700.034.9068831
TATCAAC46250.034.2454872
CTTATAG400.002793425233.988282
ATCAACG48750.032.471773
AACGCAG49450.032.4207846
TCAACGC49150.032.414854
TACTTAT1102.7970673E-432.18297670
CAACGCA49950.031.9602495
ACGCAGA51750.031.0454947
CGCAGAG52100.030.7717428
AGAGTAC52700.030.54865311
CAGAGTA53050.030.4094910
GCAGAGT53200.030.3270239
GACAGGT1800.030.2118051
GATCTTA450.004988489730.1922367
Produced by FastQC (version 0.11.5)