FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263782
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATACAGCTCCAACAATAAGAACTACCTAGCTTGGTACCAGCAGAA13150.4985177153861901No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12080.4579539164916484No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT9740.36924430021760396No Hit
ATACTATAGTTCTCCGCTCACTTTCGGCGGAGGGACCCAGGTGGAGATCAAACGAACTGTGGCTGCACCAT9480.35938767618715456No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9410.35673396971741816No Hit
GTCCCAGACCGATTCAGTGGCGGCGGGTCTGGGACAGATTTCACTCTCAC7890.2991106292317141No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7490.2839465922617919No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6850.25968413310991656No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6440.24414099521574634No Hit
CTATAGTATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC6160.23352616933680084No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG5910.2240486462305995No Hit
ACTTAGGAGCCTGTCCGGGTTTCTGCTGGTACCAAGCTAGGTAGTTCTTATTGTTGGAGCTGTATAAAAGACT5870.2225322425336073No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT5590.21191741665466182No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG5550.2104010129576696No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5050.19144596674526693No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4980.18879226027553056No Hit
GTTTATTACTGTCAACAATACTATAGTTCTCCGCTCACTTTCGGCGGAGGGACCCAGGTGGAGATCAAACGAA4780.18121024179056947No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4590.17400732422985646No Hit
CTCCTAAGTTGCTCATTTCTTGGGCTTCTGCCCGGCAATCCGGGGTCCCA4420.16756260851763957No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT4360.16528800297215124RNA PCR Primer, Index 29 (95% over 23bp)
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCCGCCA4330.16415070019940708No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATACTATAGTTCT4290.16263429650241487No Hit
GTCTGGGACCCCGGATTGCCGGGCAGAAGCCCAAGAAATGAGCAACTTAGGAGCCTGTCCGGGTTTCTGCTGGTA4270.16187609465391878No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT4220.1599805900326785No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT4100.15543137894170186No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4090.1550522780174538No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4060.15391497524470965No Hit
GTATAAAAGACTCTGGCTGGACTTGCAATTGATGGTGGCCCTCTCGCCCAGAGACGCGGCCAGGGAGTCTGGAGA4060.15391497524470965No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA3920.1486075623052369No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC3880.14709115860824468No Hit
CTATAGTTCTCCGCTCACTTTCGGCGGAGGGACCCAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT3810.1444374521385083No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA3770.1429210484415161No Hit
ACCTAGCTTGGTACCAGCAGAAACCCGGACAGGCTCCTAAGTTGCTCATT3550.13458082810805894No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC3520.13344352533531476No Hit
GAACTATAGTATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT3520.13344352533531476No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA3520.13344352533531476No Hit
GTCCCAGACCCGCCGCCACTGAATCGGTCTGGGACCCCGGATTGCCGGGC3460.13116891978982645No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG3410.12927341516858618No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG3400.12889431424433812No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA3360.12737791054734593No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA3330.12624060777460175No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC3240.12282869945636928No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT3170.12017499298663291No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT3150.1194167911381368No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT3040.11524668097140821No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG3030.11486758004716016No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG2980.11297207542591987No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC2960.11221387357742377No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT2960.11221387357742377No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG2950.11183477265317573No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC2940.11145567172892769No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG2930.11107657080467961No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC2900.10993926803193547No Hit
AAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTC2880.10918106618343937No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC2850.10804376341069519No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTGGGTCCCTCCGCCGAAAGTG2850.10804376341069519No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTGGGTCC2840.10766466248644715No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA2750.10425275416821465No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC2730.10349455231971855No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT2660.10084084584998218No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT2650.10046174492573413No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG2640.10008264400148607No Hit
GTCATGACCCAGTCTCCAGACTCCCTGGCCGCGTCTCTGGGCGAGAGGGC2640.10008264400148607No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)