FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263782
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40831.5478690737048015No Hit
CTTTTATACAGCTCCAACAATAAGAACTACCTAGCTTGGTACCAGCAGAA11730.44468538414296654No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10840.4109454018848898No Hit
ATACTATAGTTCTCCGCTCACTTTCGGCGGAGGGACCCAGGTGGAGATCAAACGAACTGTGGCTGCACCAT8480.32147758376234925No Hit
GTCCCAGACCGATTCAGTGGCGGCGGGTCTGGGACAGATTTCACTCTCAC7610.2884958033527686No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6150.23314706841255278No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6060.22973516009432032No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT5670.21495022404864622No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT5580.21153831573041373No Hit
CTATAGTATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC5560.21078011388191764No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG5360.20319809539695657No Hit
GTTTATTACTGTCAACAATACTATAGTTCTCCGCTCACTTTCGGCGGAGGGACCCAGGTGGAGATCAAACGAA4860.18424304918455392No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT4860.18424304918455392No Hit
CTCCTAAGTTGCTCATTTCTTGGGCTTCTGCCCGGCAATCCGGGGTCCCA4790.18158934271481755No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC4770.18083114086632143No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC4730.17931473716932922No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG4290.16263429650241487No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA4210.15960148910843044No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG4100.15543137894170186No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCCGCCA3930.14898666322948495No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT3810.1444374521385083No Hit
ACTTAGGAGCCTGTCCGGGTTTCTGCTGGTACCAAGCTAGGTAGTTCTTATTGTTGGAGCTGTATAAAAGACT3770.1429210484415161No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT3770.1429210484415161No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA3750.14216284659301998No Hit
GAACTATAGTATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT3670.13913003919903555No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG3640.1379927364262914No Hit
CTATAGTTCTCCGCTCACTTTCGGCGGAGGGACCCAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT3540.13420172718381088No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC3530.13382262625956282No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA3530.13382262625956282No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATACTATAGTTCT3520.13344352533531476No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT3500.13268532348681866No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT3380.12813611239584202No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG3350.12699880962309787No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT3320.12586150685035372No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA3310.12548240592610566No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGCTGCTCTGGATCTCTGGAAC3270.12396600222911343No Hit
GTCCCAGACCCGCCGCCACTGAATCGGTCTGGGACCCCGGATTGCCGGGC3270.12396600222911343No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC3220.12207049760787317No Hit
GTCATGACCCAGTCTCCAGACTCCCTGGCCGCGTCTCTGGGCGAGAGGGC3170.12017499298663291No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC3080.11676308466840042No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA3060.11600488281990431No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG2990.11335117635016795No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC2980.11297207542591987No Hit
GTCTCCAGACTCCCTGGCCGCGTCTCTGGGCGAGAGGGCCACCATCAATTGCAAGTCCAGCCAGAGTCTTTTATA2970.11259297450167184No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC2950.11183477265317573No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT2920.11069746988043158No Hit
GTCTGGGACCCCGGATTGCCGGGCAGAAGCCCAAGAAATGAGCAACTTAGGAGCCTGTCCGGGTTTCTGCTGGTA2900.10993926803193547No Hit
ACCTAGCTTGGTACCAGCAGAAACCCGGACAGGCTCCTAAGTTGCTCATT2850.10804376341069519No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT2850.10804376341069519No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG2760.10463185509246271No Hit
ATTCAGTGGCGGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC2740.10387365324396661No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA2740.10387365324396661No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA2730.10349455231971855No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA2660.10084084584998218No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTGGGTCCCTCCGCCGAAAGTG2660.10084084584998218No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC2640.10008264400148607No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)