FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1323068
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46550.35183376818122725No Hit
GTATTACTGTCAGCAGTATGGTTTCTCATCTTGGACGTTCGGCCAAGGGA33620.2541063649033912No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23490.17754189504998988No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA18900.14284980061493438No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17330.13098344151623348No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15940.12047755670910337No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15850.11979731956331797No Hit
GTGTATTACTGTCAGCAGTATGGTTTCTCATCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA15840.11972173765823071No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA14530.10982050809179877No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14270.10785537855952981No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13290.10044835186097767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15850.039.9038281
GTATCAA40600.038.191761
AACTGTG20100.032.10062470
TATCAAC49450.031.2061142
ATCAACG49950.031.0204453
TCAACGC49900.030.9728894
CAACGCA51000.030.3002645
CGAATGT450.00498242430.20069315
AACGCAG51700.030.0213936
ACGCAGA54250.028.5476467
CGCAGAG54700.028.3127948
AGAGTAC56250.027.3483811
GCAGAGT57650.026.9808089
TATTCCG957.334007E-626.62677464
GTGGTAT8000.026.3531551
CGTTCGG14500.026.23825636
CAGAGTA60550.025.74369610
GGACGTT15150.025.27800633
CGAACTG14800.024.75141168
GAACTGT16900.024.50778469
Produced by FastQC (version 0.11.5)