FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491728
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT23590.4797367650408356No Hit
CTATTATGCTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC14970.30443659909543486No Hit
GCATAATAGTTACCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT12750.25928968860833634No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT11450.23285230859336872No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA11340.23061529951517915No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11280.22939511274525753No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG7980.1622848403995705No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7890.16045456024468813No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7780.15821755116649855No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7430.15109979500862264No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7300.14845605700712589No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7210.14662577685224354No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGCAATTATTTAGCCTGGTTTCAGCAGAAA6730.13686428269287088No Hit
GTAATAGACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA6680.13584746038460285No Hit
GCATAGATCAGGCGCTTAGGGACTTTCCCTGGTTTCTGCTGAAACCAGGC6340.12893306868838056No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6140.12486577945530863No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT6070.12344222822373344No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG5710.11612110760420395No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC5560.1130706406794No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC5540.1126639117560928No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC5370.10920671590798166No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT5180.1053427911365633No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT5150.1047326977516025No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC5150.1047326977516025No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC5070.10310578205837374No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG5060.10290241759672014No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5040.10249568867341295No Hit
ACTTATTACTGTCTACAGCATAATAGTTACCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA5000.10168223082679854No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT4960.10086877298018417No Hit
ATAGTTACCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA4930.10025867959522337No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG252.5262596E-455.3731341
ACGATCG200.00835848450.10510322
GGTATCA5850.049.636431
TCGTATG5350.047.07842340
CTTATAC6600.047.030131
AGACCGG6600.045.6290525
CGTATGC5550.044.8971341
GCCTATC5500.044.84670333
TTATACA7100.044.658322
GGATAGG307.4257207E-444.5016256
GACCGGA6800.044.30960526
TATCTCG5150.044.27713436
CCCACGA7150.043.8926219
ACCGGAG6800.043.85307327
ACGAGAC6950.043.73682822
AGCCTAT6650.043.6067532
GAGCCTA6900.043.40365231
CGGAGCC7050.043.32814429
CGTTTGC404.9060574E-543.26025441
CCTATCT5350.043.06599834
Produced by FastQC (version 0.11.5)