FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1319250
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42020.3185143073716127No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37920.28743604320636723No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36840.27924957362137576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35950.2725033162781884No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31690.24021224180405532No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG29560.2240667045669888No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29390.2227780936137957No Hit
ATATTACTGTCAACCGTATGATAATTTTTCCCTACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA28200.21375781694144402No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27560.2089065757058935No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26990.2045859389804813No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25140.19056281978396816No Hit
GTTGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATGAGCAACTATTTGAATTGGTATCAGCAGAAA24460.18540837597119575No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24030.18214894826606026No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23880.1810119386014781No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTACTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA23450.1777525108963426No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22280.16888383551260186No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21760.16494220200871706No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21660.1641841955656623No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21620.16388099298844042No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATGAGCAA21100.15993935948455562No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTACTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA21030.15940875497441728No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20640.1564525298465037No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG20500.155391320826227No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20360.15433011180595035No Hit
ACATATTACTGTCAACCGTATGATAATTTTTCCCTACTCACTTTCGGCGG19950.1512222853894258No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18090.13712336554860716No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17850.13530415008527572No Hit
TCAGTAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACCGTAT17580.13325753268902787No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG17240.13068031078264164No Hit
GATCTACGATGCGTCCATTTTGGAAACAGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTA16870.127875686943339No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16700.1265870759901459No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC16400.12431305666098162No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16330.12378245215084328No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16010.12135683153306803No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC15860.12021982186848588No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15820.11991661929126399No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT15760.1194618154254311No Hit
AGTCAGGACATGAGCAACTATTTGAATTGGTATCAGCAGAAACCAGGGAA15660.11870380898237635No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15060.11415577032404776No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14970.11347356452529847No Hit
GGCTACTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCACTGAAC14930.11317036194807657No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14780.1120333522834944No Hit
GATTTTACTTTCACCATCAGTAGCCTGCAGCCTGAAGATATTGCAACATA14540.11021413682016296No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT14460.10960773166571916No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14440.1094561303771082No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14310.10847072200113701No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14310.10847072200113701No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14130.10710631040363842No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13660.10354368012128104No Hit
TCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT13600.10308887625544817No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13290.10073905628197839No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5300.047.1304861
CGATTGT750.001741850637.42077670
GTATCAA43000.036.8171771
TTATACG400.002744116134.11301435
TATCAAC49550.031.7302932
ATCAACG51200.030.6914313
TACGAAT450.00473486130.5170138
TCAACGC51800.030.3290314
CAACGCA53050.029.6143995
AACGCAG53800.029.3884356
CGCAGAG56250.028.0484778
ACGCAGA56400.028.0336537
AGAGTAC57000.027.61713211
CAGAGTA57600.027.27299910
GCAGAGT58800.026.7737399
GATTCAC4200.025.7185671
TGTTAGA18550.025.3422470
CACTCGT2950.024.33448258
CACTTTA4550.023.7105125
GCTCAAT750.001699895223.63790353
Produced by FastQC (version 0.11.5)