FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1235796
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC35930.290743779717688No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAAGAACTCAGGCTGGTGTCATAAGA35880.29033918219511956No Hit
TCACTGGGCTGTGGGCTGAGGATGAGGCTGATTATTACTGCCAGTCTTAT34850.2820044732302095No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTTCACTGGTACCAGCAGGTTCCAGGAACAGC33240.26897643300350543No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31980.2587805754347805No Hit
GCCCAGTGATGGCCAGGGAGGCTGAAGTGCCAGACTTGGAGCCAGAGAAT27710.2242279470074349No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27660.22382334948486643No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27420.22188128137653784No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26880.2175116281327986No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAAGAACTCAGG25840.20909599966337486No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA24980.20213692227519753No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTACCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG23290.18846152601238392No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22650.18328267772350776No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC22550.18247348267837088No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTACCTTTT22420.1814215291196929No Hit
GGTTATGATGTTCACTGGTACCAGCAGGTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAACAAGCGA22160.17931762200233695No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22040.17834658794817268No Hit
CTTCATGCGTGACCTGGCAGCTGTACCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG21730.1758380833082483No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21420.17332957866832388No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC20900.16912176443361202No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC20370.16483303069438646No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20190.16337647961314003No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19410.15706475826107222No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19400.15698383875655852No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19370.15674108024301747No Hit
GTCTTATGACACCAGCCTGAGTTCTTCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG19060.15423257560309309No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18870.15269510501733297No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18660.15099579542254546No Hit
GTGATGGCCAGGGAGGCTGAAGTGCCAGACTTGGAGCCAGAGAATCGGTC18650.1509148759180318No Hit
CCCTTCATGCGTGACCTGGCAGCTGTACCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18410.1489728078097032No Hit
GATTATTACTGCCAGTCTTATGACACCAGCCTGAGTTCTTCGGTGTTCGG17790.14395579852985446No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17370.14055717934027948No Hit
GGCCATCACTGGGCTGTGGGCTGAGGATGAGGCTGATTATTACTGCCAGT17140.13869603073646458No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16730.1353783310514033No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC15890.12858109267225334No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15690.12696270258197956No Hit
GGATGAGGCTGATTATTACTGCCAGTCTTATGACACCAGCCTGAGTTCTTCGGTGTTCGGCGGAGGGACCAAG15470.12518247348267839No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15180.12283580785178137No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC15130.12243121032921292No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAAGAACTCAGGC15090.12210753231115816No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14900.12057006172539804No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14640.11846615460804209No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14390.11644316699519984No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTTCACTGGTACCAG13990.11320638681465225No Hit
GTCATAAGACTGGCAGTAATAATCAGCCTCATCCTCAGCCCACAGCCCAG13900.11247811127402904No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13850.11207351375146059No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTACCTTTTGT13800.11166891622889213No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13390.10835121654383087No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13050.1055999533903654No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12730.10301052924592732No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC12590.10187765618273567No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG12590.10187765618273567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17750.044.7481161
GTGGTAT4950.042.0089531
TGGTATC5100.039.436562
GTATCAA45400.038.8947641
GACCGGA901.0281681E-438.1595270
CGTATGC2050.037.06200841
TCGTATG2000.036.2039240
TATCAAC54000.032.9529232
GTATGCC2500.031.83097342
TCTCGTA1850.031.6001838
ATCAACG56650.031.351263
TCAACGC56850.031.1197834
AACGCAG57850.030.817546
CAACGCA58050.030.6526415
ATGCCGT2200.029.98183344
CCCACGA3200.029.83049419
CGTCTTC2350.029.70327848
CACGAGA3100.029.6906221
AGGGGGG4000.029.1920370
CCCTAAG3050.029.10914828
Produced by FastQC (version 0.11.5)