FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1235796
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC34260.27723022246390183No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTTCACTGGTACCAGCAGGTTCCAGGAACAGC32590.2637166652101156No Hit
TCACTGGGCTGTGGGCTGAGGATGAGGCTGATTATTACTGCCAGTCTTAT32550.26339298719206083No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30970.2506077054788978No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30850.24963667142473353No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAAGAACTCAGGCTGGTGTCATAAGA26990.2184017426824492No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26250.21241369934843615No Hit
GGTTATGATGTTCACTGGTACCAGCAGGTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAACAAGCGA24100.1950160058779928No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23990.1941258913283422No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23990.1941258913283422No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23520.19032267461619878No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTACCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22770.18425371177767205No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG22390.18117877060615184No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22050.17842750745268637No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTACCTTTT21820.1765663588488715No Hit
CTTCATGCGTGACCTGGCAGCTGTACCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG20460.16556130623500967No Hit
GCCCAGTGATGGCCAGGGAGGCTGAAGTGCCAGACTTGGAGCCAGAGAAT20300.16426659416279063No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19550.15819763132426387No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19300.15617464371142162No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19050.15415165609857936No Hit
GTCTTATGACACCAGCCTGAGTTCTTCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG19040.15407073659406567No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC18930.1531806220444151No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18800.15212866848573714No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17930.1450886715930461No Hit
GATTATTACTGCCAGTCTTATGACACCAGCCTGAGTTCTTCGGTGTTCGG17520.1417709719079848No Hit
CCCTTCATGCGTGACCTGGCAGCTGTACCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG16910.13683488213264972No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC16540.1338408604656432No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC16190.13100867780766404No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC15780.12769097812260274No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAAGAACTCAGG15570.12599166852781526No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15370.12437327843754147No Hit
GGCCATCACTGGGCTGTGGGCTGAGGATGAGGCTGATTATTACTGCCAGT15260.12348316388789088No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15240.1233213248788635No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15240.1233213248788635No Hit
GGATGAGGCTGATTATTACTGCCAGTCTTATGACACCAGCCTGAGTTCTTCGGTGTTCGGCGGAGGGACCAAG15000.12137925677053495No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14730.1191944301486653No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC14630.11838523510352841No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14460.11700960352679568No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTTCACTGGTACCAG14410.11660500600422724No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC14070.11385374285076179No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14010.11336822582367963No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC13910.11255903077854275No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13410.10851305555285824No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT13400.10843213604834455No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13350.1080275385257761No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13150.1064091484355023No Hit
GTGATGGCCAGGGAGGCTGAAGTGCCAGACTTGGAGCCAGAGAATCGGTC12930.10462891933620112No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12880.10422432181363267No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG12840.10390064379557791No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC12420.10050202460600292No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)