FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences952550
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG55310.580651934281665No Hit
GATTACTACTGTGCAGCATGGGATGACAGCCTGAGTGTTTGGGTGTTCGG50760.532885412839221No Hit
ACCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC32420.3403495879481392No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28050.2944727310902315No Hit
GCCTTGGGTTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAACACTCAGGCTGTCATCCCATG27960.29352789879796337No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25600.268752296467377No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22660.23788777491995172No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGTTGACCTAGGACGGTCACCTTGGTCCCTC21490.22560495512046613No Hit
CCCAAACACTCAGGCTGTCATCCCATGCTGCACAGTAGTAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGG20960.22004094273266497No Hit
GTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCC20700.21731142722166813No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20230.2123773030287124No Hit
GTCCTAGGTCAACCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC19440.2040837751299144No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA18790.19725998635242245No Hit
GTAATACACACCCGTGTCCTCGGCTCTCAGGCTCTTCATCTGCAGAAACA18770.1970500236208073No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG18630.19558028449950132No Hit
AGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAACCA18560.19484541493884835No Hit
CTACTGTGCAGCATGGGATGACAGCCTGAGTGTTTGGGTGTTCGGCGGAG18300.19211589942785154No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18070.18970132801427744No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18030.1892814025510472No Hit
GTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC18030.1892814025510472No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAACACTCAGGC17950.18844155162458665No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17850.18739173796651093No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17690.18571203611358983No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG17360.18224765104194005No Hit
GTGTTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAACCC17300.18161776284709466No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17180.1803579864574038No Hit
GATTATTCCTATACATGAGCAGTTTGGGGGCCGTTCCTGGGAGGTGCTGA16710.17542386226444806No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16320.17132958899795286No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16250.17059471943729987No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCT16020.16818014802372577No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15810.16597553934176684No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15580.16356096792819275No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCAC15130.15883680646685214No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15030.15778699280877645No Hit
GTTTGGGGGCCGTTCCTGGGAGGTGCTGATACCAGTATACATAATTAGTT14470.15190803632355257No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14380.15096320403128446No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14360.1507532412996693No Hit
GCACAGTAGTAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAG14340.1505432785680542No Hit
GCATGGGATGACAGCCTGAGTGTTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAACCCAAGG14300.1501233531048239No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA14200.1490735394467482No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14200.1490735394467482No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC13970.1466589680331741No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT13470.14140989974279566No Hit
CCCCTGAGGGCCTCTGATTATTCCTATACATGAGCAGTTTGGGGGCCGTTCCTGGGAGGTGCTGATACCAGTATA13460.14130491837698808No Hit
GGAATAATCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG13350.14015012335310484No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGTTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACAC13260.1392052910608367No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12850.13490105506272637No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC12840.1347960736969188No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12520.1314366699910766No Hit
CCTTGGTCCCTCCGCCGAACACCCAAACACTCAGGCTGTCATCCCATGCTGCACAGTAGTAATCAGCCTCATCCT12240.12849719174846463No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12190.1279722849194268No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTACTACTGTGCAG12150.12755235945619653No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC12020.12618760170069812No Hit
GAGTGACCGAGGGGGCAGCCTTGGGTTGACCTAGGACGGTCACCTTGGTC11580.12156842160516508No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC11480.12051860794708938No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC11430.11999370111805154No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG11400.11967875702062884No Hit
GCCCCCAAACTGCTCATGTATAGGAATAATCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT11390.11957377565482126No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11210.11768411107028502No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11130.11684426014382447No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10750.11285496824313686No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG10590.11117526639021573No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT10540.11065035956117789No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG10510.1103354154637552No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAACACTCAGG10240.10750091858695082No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT10210.10718597448952812No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10180.1068710303921054No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10080.1058212167340297No Hit
CTCTTGTTCTGGAACCACCTCCAACATCGGAACTAATTATGTATACTGGTATCAGCACCTCCCAGGAACGGCCCC10030.10529630990499188No Hit
ACCACATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCACTGTTTCTG10030.10529630990499188No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC10010.10508634717337673No Hit
CAGTAATACACACCCGTGTCCTCGGCTCTCAGGCTCTTCATCTGCAGAAA9980.10477140307595402No Hit
GTGTATTACTGTGCGAGAGATTGGTCCTCACCTATAGCAGTGGCGAATGG9930.10424649624691618No Hit
GGACCAAGGTGACCGTCCTAGGTCAACCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG9920.10414151488110861No Hit
GTACAGACTGGGCCCAGGACCCTGCACAGTGAGTGAGGAGGGTGAGGAGG9910.10403653351530102No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9880.10372158941787833No Hit
TTCCTATACATGAGCAGTTTGGGGGCCGTTCCTGGGAGGTGCTGATACCA9880.10372158941787833No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9880.10372158941787833No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9780.10267177575980263No Hit
GTATGTGGTAGTACTAGTACTGCTAATGTATGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTG9720.1020418875649572No Hit
GGATGACAGCCTGAGTGTTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAACCCAAGGCTGCCCC9550.10025720434622853No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)