FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185664
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41180.34731593436251756No Hit
GAGTTACACTACCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTT41050.3462195023210623No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40000.3373637050631545No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30270.2552999838065422No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29040.24492604987585015No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28490.24028729893123177No Hit
GTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAATTCTGTC28290.23860048040591603No Hit
GATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCA28280.23851613947965022No Hit
ACCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG27360.23075677426319768No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27270.22999770592680557No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG26040.21962377199611355No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24390.20570751916225843No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23150.19524924430530066No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23060.19449017596890858No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22970.19373110763251647No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCACCTATTTAAATTGGTATCAGCAGAAA22880.19297203929612436No Hit
ACACTACCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACT22760.1919599481809349No Hit
GAATTCACTCTCACCATCAGCGGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACACTACC22650.19103219799201124No Hit
GCATAGATCAGGACTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT22040.18588740148979813No Hit
GGGTAGTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAATT21700.18301980999676132No Hit
GGTGAGGGTAGTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAG21100.17795935442081398No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20820.1755978084853719No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG19530.16471782899708518No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19510.1645491471445536No Hit
GTCCTGATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTT19270.16252496491417467No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19250.1623562830616431No Hit
GATCAGGACTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT19010.16033210083126415No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18950.15982605527366944No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGGGGTGCCAGAGGTGACAT18520.15619939544424052No Hit
CTGCTACTCTGGCTCCGGGGTGCCAGAGGTGACATCCAGATGACCCAGTC18360.1548499406239879No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17770.14987382597430637No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17600.14844003022778796No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17480.14742793911259852No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17260.14557243873475115No Hit
GTAATACATGGCCGTGTCCGCGGCGGTCACAGAGTTCACTTTCAGGGAGA16350.13789741444456438No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16340.1378130735182986No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC16070.13553586850912233No Hit
ACTTACTACTGTCAACAGAGTTACACTACCCTCACCTTCGGCCAAGGGAC16000.1349454820252618No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA15810.1333430044262118No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15700.13241525423728812No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15550.1311501403433013No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCA14930.12592100291482242No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14790.12474022994710136No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14580.1229690704955198No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14520.12246302493792507No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14510.1223786840116593No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC14050.118499001403433No Hit
CACCATCAGCGGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACACTACCCTCACCTTCGG14040.11841466047716723No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14040.11841466047716723No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13690.11546272805786462No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGGGGTGCCAGAGGTGACATCCAGATGACCCAGTCTCCCTCCTCC13080.1103179315556515No Hit
CCCTCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG12980.10947452229299363No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA12930.10905281766166468No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12870.10854677210406995No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12790.10787204469394364No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT12590.10618522616862788No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12440.10492011227464106No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12420.10475143042210948No Hit
GTCTCCCTCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAC12380.10441406671704632No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12040.1015464752240095No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12010.10129345244521214No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11880.10019702040375687No Hit
CTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGGTGCTA11870.1001126794774911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT652.1645974E-1047.71282239
GTGGTAT2750.045.8939821
CGTATGC1600.045.3224241
TGTCGTA704.5474735E-1044.23501238
TCGTATG1650.043.89839640
GTATCAA28700.038.7456051
ATGTCGT801.7007551E-938.67273337
CGATGTC901.2914825E-1038.1417835
TACTGAG8350.036.363870
GAGCGAT2250.035.00276632
TGAGCGA2450.034.92290531
GCTATAC400.002745616534.1089553
CGAGACA2750.033.49868423
CACGAGA2550.033.44579721
TCTCCGA2600.032.78877611
TATCAAC33600.032.6877482
GATGTCG959.1840775E-932.54022636
CTTATAC2550.032.10391
ATCAACG34600.031.8415973
TCAACGC34850.031.711054
Produced by FastQC (version 0.11.5)