FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185664
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40230.3393035463672676No Hit
GAGTTACACTACCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTT38170.32192931555651516No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31050.2618785760552737No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28830.24315489042426858No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27430.2313471607470582No Hit
GTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAATTCTGTC26890.2267927507287056No Hit
GATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCA26030.21953943106984777No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG25730.21700920328187415No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25370.21397292993630573No Hit
ACCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG24330.20520147360466373No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23180.195502267084098No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22560.19027312965561913No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22490.18968274317175862No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCACCTATTTAAATTGGTATCAGCAGAAA21800.18386321925941917No Hit
GGTGAGGGTAGTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAG20860.17593517219043506No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20810.17551346755910613No Hit
GTCCTGATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTT20800.17542912663284033No Hit
ACACTACCCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACT20670.1743326945913851No Hit
GGGTAGTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAATT20510.17298323977113245No Hit
GCATAGATCAGGACTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT20300.1712120803195509No Hit
GAATTCACTCTCACCATCAGCGGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACACTACC20000.16868185253157725No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19520.16463348807081937No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19200.16193457843031414No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18480.15586203173917737No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18310.15442823599265898No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18000.15181366727841952No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17840.1504642124581669No Hit
GATCAGGACTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT17660.1489460757853827No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17380.14658452984994064No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17370.14650018892367483No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG16860.1421988016841196No Hit
CTGCTACTCTGGCTCCGGGGTGCCAGAGGTGACATCCAGATGACCCAGTC16790.1416084152002591No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16440.1386564827809565No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16080.1356202094353881No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA16010.1350298229515276No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGGGGTGCCAGAGGTGACAT15880.13393339091007234No Hit
ACTTACTACTGTCAACAGAGTTACACTACCCTCACCTTCGGCCAAGGGAC15690.13233091331102234No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC15330.12929463996545396No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15190.12811386699773292No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14710.12406550253697506No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACCGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCA14360.12111357011767246No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14120.11908938788729354No Hit
GTAATACATGGCCGTGTCCGCGGCGGTCACAGAGTTCACTTTCAGGGAGA14110.11900504696102776No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13920.11740256936197777No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13340.11251079563856202No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC13230.11158304544963835No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA13210.11141436359710677No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13210.11141436359710677No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13120.11065529526071466No Hit
CACCATCAGCGGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACACTACCCTCACCTTCGG12860.10846243117780417No Hit
CCCTCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG12850.10837809025153837No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCACT12810.10804072654647522No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12400.1045827485695779No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12270.10348631652812264No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12190.10281158911799634No Hit
CTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGGTGCTA12000.10120911151894635No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11970.10095608874014898No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11950.1007874068876174No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)