FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784959
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG51180.6520085762441096No Hit
GATTACTACTGTGCAGCCTGGGATGACAGCCTGAGTGTTTGGGTGTTCGG47130.6004135247828231No Hit
ACCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC28890.3680447004238438No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27870.35505039116692716No Hit
GCCTTGGGTTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAACACTCAGGCTGTCATCCCAGG24200.30829635687978607No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21950.27963243940129356No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21590.2750462126047348No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGTTGACCTAGGACGGTCACCTTGGTCCCTC20230.257720466928846No Hit
GTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCC20040.25529995834177327No Hit
CCCAAACACTCAGGCTGTCATCCCAGGCTGCACAGTAGTAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGG18990.24192346351847677No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT17980.22905654945035345No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17330.22077586217878897No Hit
GTCCTAGGTCAACCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC17280.22013888623482245No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17220.21937451510206266No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16920.21555265943826366No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG16800.21402391717274405No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16720.21300475566239765No Hit
AGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAACCA16610.21160340858567134No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16480.20994727113135847No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAACACTCAGGC16460.20969248075377186No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA16010.20395969725807336No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15880.20230355980376044No Hit
CTACTGTGCAGCCTGGGATGACAGCCTGAGTGTTTGGGTGTTCGGCGGAG15850.20192137423738055No Hit
GTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC15840.20179397904858723No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15830.20166658385979394No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCT15800.20128439829341405No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT15730.20039263197186097No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15650.19937347046151455No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15110.19249413026667636No Hit
GTGTTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAACCC15100.19236673507788304No Hit
GATTATCCCTATACATGAGCAGTTTGGGGGCCGTTCCTGGGAGGTGCTGA14360.18293949110717886No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG14010.17848065949941336No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13690.17440401345802772No Hit
GCACAGTAGTAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAG13490.17185610968216175No Hit
GGGATAATCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG13120.16714248769680964No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13050.16625072137525654No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA12950.16497676948732354No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12470.15886180042524514No Hit
GTTTGGGGGCCGTTCCTGGGAGGTGCTGATACCAGTATACATAATTAGTT12400.15797003410369204No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12320.15695087259334564No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCAC12040.15338380730713325No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12020.15312901692954664No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC11840.15083590353126725No Hit
GTAATACACACCCGTGTCCTCGGCTCTCAGGCTCTTCATCTGCAGAAACA11620.14803320937781464No Hit
CCCCTGAGGGCCTCTGATTATCCCTATACATGAGCAGTTTGGGGGCCGTTCCTGGGAGGTGCTGATACCAGTATA11430.14561270079074193No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGTTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACAC11420.14548530560194864No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC10770.13720461833038414No Hit
GCCTGGGATGACAGCCTGAGTGTTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAACCCAAGG10460.13325536747779185No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC10400.13249099634503203No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT10290.13108964926830574No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9990.1272677936045067No Hit
CCTTGGTCCCTCCGCCGAACACCCAAACACTCAGGCTGTCATCCCAGGCTGCACAGTAGTAATCAGCCTCATCCT9980.12714039841571345No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC9890.1259938417165737No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9870.12573905133898713No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9870.12573905133898713No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG9730.12395551869588094No Hit
CTCTTGTTCTGGAACCACCTCCAACATCGGAACTAATTATGTATACTGGTATCAGCACCTCCCAGGAACGGCCCC9680.12331854275191444No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9650.12293635718553453No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC9610.12242677643036133No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG9520.12128021973122163No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC9500.12102542935363504No Hit
GCCCCCAAACTGCTCATGTATAGGGATAATCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT9370.11936929189932212No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9310.11860492076656232No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9250.11784054963380254No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9200.11720357368983604No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9110.11605701699069634No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT9090.11580222661310972No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAACACTCAGG9020.11491046029155665No Hit
GTATAGGGATAATCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC8960.11414608915879683No Hit
GAGTGACCGAGGGGGCAGCCTTGGGTTGACCTAGGACGGTCACCTTGGTC8950.11401869397000353No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC8830.11248995170448392No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTACTACTGTGCAG8750.11147079019413753No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGTTGAC8400.10701195858637202No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC8350.10637498264240552No Hit
TCCCTATACATGAGCAGTTTGGGGGCCGTTCCTGGGAGGTGCTGATACCA8310.10586540188723233No Hit
GGACCAAGGTGACCGTCCTAGGTCAACCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG8290.10561061150964574No Hit
ATCCCAGGCTGCACAGTAGTAATCAGCCTCATCCTCGGACCGGAGCCCAC8290.10561061150964574No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC8230.10484624037688593No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG8190.10433665962171274No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC8170.10408186924412613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3400.054.67655670
CGCGAAC200.006141863354.16884664
GCGCGAA200.006212868754.0116963
CCTTCGT200.00775766751.06252727
ACGTTCG753.456514E-545.90130270
TTATACA4200.043.7121162
CTTATAC4200.043.7121161
GCCAGAG20250.043.18122570
TATACAC4450.042.0203863
GTGGTAT2300.039.9110641
GCTAATC3700.039.7317633
CGTATGC4200.039.4917341
CGCTAAT4250.039.38119532
TCGTATG4150.039.06910340
TCGCTAA4650.038.88950331
ATGCCGT4200.038.8576944
GTACGGA350.00144815738.8552131
ACTCGCT4650.038.82496329
ATCTCCG4750.038.6457910
ATCTCGT3500.038.23950637
Produced by FastQC (version 0.11.5)