FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784959
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTACTACTGTGCAGCCTGGGATGACAGCCTGAGTGTTTGGGTGTTCGG43060.54856368294395No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41350.5267791056602956No Hit
ACCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC30620.3900840680850847No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26960.3434574289867369No Hit
GTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCC23680.3016718070625345No Hit
AGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAACCA22790.2903336352599308No Hit
GTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC19880.2532616353210804No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG19490.24829322295814174No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19330.24625489993744895No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCT19180.24434397210554948No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT18120.2308400820934597No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG18080.23033050133828645No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA17940.22854696869518026No Hit
GTGTTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAACCC17700.22548948416414105No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA17450.22230460444430858No Hit
GCCTTGGGTTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAACACTCAGGCTGTCATCCCAGG16880.21504307868309047No Hit
GTCCTAGGTCAACCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC16430.20931029518739194No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16330.20803634329945894No Hit
GGGATAATCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG16070.20472406839083315No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15470.19708035706323515No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15180.19338589658822944No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15140.19287631583305626No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14430.18383125742873194No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCAC14270.18179293440803915No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGTTGACCTAGGACGGTCACCTTGGTCCCTC13790.17567796534596072No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13610.17338485194768133No Hit
CTACTGTGCAGCCTGGGATGACAGCCTGAGTGTTTGGGTGTTCGGCGGAG13550.17262048081492154No Hit
GTTTGGGGGCCGTTCCTGGGAGGTGCTGATACCAGTATACATAATTAGTT13380.17045476260543543No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA13070.16650551175284314No Hit
GATTATCCCTATACATGAGCAGTTTGGGGGCCGTTCCTGGGAGGTGCTGA12800.16306584165542404No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12760.16255626090025085No Hit
GTAGTAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG12750.16242886571145754No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12730.16217407533387093No Hit
CTCTTGTTCTGGAACCACCTCCAACATCGGAACTAATTATGTATACTGGTATCAGCACCTCCCAGGAACGGCCCC12560.16000835712438485No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12520.15949877636921164No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG12420.15822482448127864No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12150.15478515438385954No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC11720.14930716126574764No Hit
GTATAGGGATAATCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC11500.14650446711229503No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGTTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACAC11310.14408395852522235No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG11070.14102647399418314No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11020.14038949805021664No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC11010.14026210286142332No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT10990.14000731248383674No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC10890.13873336059590374No Hit
GCCTGGGATGACAGCCTGAGTGTTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAACCCAAGG10850.13822377984073053No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10840.13809638465193724No Hit
CCCCTGAGGGCCTCTGATTATCCCTATACATGAGCAGTTTGGGGGCCGTTCCTGGGAGGTGCTGATACCAGTATA10660.13580327125365782No Hit
GCCCCCAAACTGCTCATGTATAGGGATAATCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT10550.13440192417693153No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10160.12943351181399285No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAACACTCAGGC10080.12841435030364642No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10060.12815955992605985No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9930.12650342247174695No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT9650.12293635718553453No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9450.12038845340966854No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9310.11860492076656232No Hit
GTAATACACACCCGTGTCCTCGGCTCTCAGGCTCTTCATCTGCAGAAACA9250.11784054963380254No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9200.11720357368983604No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC9150.11656659774586953No Hit
TGTATAGGGATAATCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC8990.11452827472517672No Hit
GGGTCACCATCTCTTGTTCTGGAACCACCTCCAACATCGGAACTAATTAT8640.11006944311741122No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG8630.10994204792861793No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA8490.10815851528551174No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT8460.10777632971913183No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG8450.10764893453033852No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC8430.10739414415275193No Hit
TCCCTATACATGAGCAGTTTGGGGGCCGTTCCTGGGAGGTGCTGATACCA8390.10688456339757874No Hit
CCCAGGAACGGCCCCCAAACTGCTCATGTATAGGGATAATCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGG8280.10548321632085243No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTACTACTGTGCAG8230.10484624037688593No Hit
GGACCAAGGTGACCGTCCTAGGTCAACCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG8220.10471884518809263No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC8200.10446405481050604No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8100.10319010292257304No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTA7960.10140657027946684No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT204.234097E-4106.27894670
TTGTACG150.0025050467.935443
GTGTACG150.002509474567.905149
GGTACGT200.004495191458.61355268
TCGCGGA200.00782072150.95807319
TTGCCGA305.544838E-447.22253456
GTGGTAT2750.044.498031
AATGGTA1903.6379788E-1241.01994370
CCGAGTT350.00115382440.6927959
GCCGAGT350.00116639140.60327558
CGGAAGG350.001426992538.97176730
GCTTGCC702.4936526E-838.8474851
CGAGTTC400.002195907835.69681560
TTTGCCG400.002301065335.35815455
GTAAGAT400.002417953735.00285749
GGTAAGA501.7250204E-434.94777348
GTATCAA26200.034.899721
GCTTGAC400.002791848733.9915471
TATCAAC26600.033.9872172
ATCAACG27350.032.912053
Produced by FastQC (version 0.11.5)