FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1429265
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCGCGACGCTCACAGAGCTCAGCTTCAGGGAGA70410.49263082773313555No Hit
GTCTATGGTGGGTCCTTCAGTGGTTCCTTCTGGAGCTGGATCCGCCAGTC56190.3931391309519228No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46890.32807072166463186No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41890.2930877059187764No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG34510.24145277467789386No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33850.23683501659944095No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33130.2317974623320378No Hit
GTATATTACTGTGCGAGGAGGGCTAGTGGCTCCGTCCGCTTCCACTTCTA31800.22249198014364027No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30680.21465578461656865No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCGTTAGCGCCTATTTAAATTGGTATCAGCAAAAA30440.21297659986076758No Hit
GATCTATGCTGCGTCCACTTTGCAAAGTGGGGTCCCACCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA29430.2059100306801048No Hit
GCATAGATCAGGAGTTCAGGGGCTTTCCCTGGTTTTTGCTGATACCAATT27960.19562502405082333No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27150.18995777549999476No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27000.18890828502761908No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26880.18806869264971854No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26390.18464035710662474No Hit
GTCTCCATCCTCCCTGACTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCGTTAGCGC26080.1824714101303817No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24820.17365569016242613No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23410.16379047972209493No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG23190.16225122702927727No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22720.15896282354916688No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22360.1564440464154653No Hit
GATCAGGAGTTCAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAAT22300.15602425022651503No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22110.15469489562817254No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21950.15357543912430516No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT21680.15168635627402896No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTC20950.14657883597513408No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC20700.1448296851878413No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTCCAAAGGGCA20450.14308053440054855No Hit
GGGTTCCAGTGCCCTTTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT20430.14294060233756511No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT20180.14119145155027235No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20120.14077165536132208No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19960.1396521988574547No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19140.13391498427513443No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGGCACTTACTACTGTCAACAGGGTTCCAGTGCC18540.1297170223856318No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18540.1297170223856318No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT18450.12908732810220638No Hit
ACCATAGACAGCGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCAATAGTCCTGCGCCCCACTGCTGTAGCTGCAC18390.1286675319132561No Hit
CTCCTGATCTATGCTGCGTCCACTTTGCAAAGTGGGGTCCCACCAAGGTT18300.12803783762983073No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGGTGGGACCCCA18050.12628868684253794No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17970.12572895859060426No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17710.12390984177181978No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17620.12328014748839437No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17580.12300028336242755No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17470.12223065701601873No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17180.1202016421027591No Hit
GTCATGGACTTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC17140.11992177797679225No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17120.11978184591380885No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16990.11887228750441661No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16910.11831255925248292No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16660.11656340846519014No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16240.1136248351425383No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC16040.11222551451270407No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15870.111036091977345No Hit
GTTCAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAATAGGCGCTA15640.10942687325303566No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15220.10648829993038379No Hit
CTGGAACCCTGTTGACAGTAGTAAGTGCCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC15140.10592857167845011No Hit
GTAGTAAGTGCCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA14960.10466918311159933No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14890.10417942089115734No Hit
ACCTTGGTGGGACCCCACTTTGCAAAGTGGACGCAGCATAGATCAGGAGTTCAGGGGCTTTCCCTGGTTTTTG14750.10319989645027339No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14720.10298999835579827No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14700.10285006629281485No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT14360.10047122122209667No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14360.10047122122209667No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14330.10026132312762155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)