FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1429265
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCGCGACGCTCACAGAGCTCAGCTTCAGGGAGA65680.4595368948375564No Hit
GTCTATGGTGGGTCCTTCAGTGGTTCCTTCTGGAGCTGGATCCGCCAGTC55540.3885913389049616No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44130.3087600969729197No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38140.2668504441093849No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35830.2506882908347997No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG34100.23858416738673374No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32540.22766946647402686No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30850.21584520715192773No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCGTTAGCGCCTATTTAAATTGGTATCAGCAAAAA27580.19296631485413832No Hit
GTATATTACTGTGCGAGGAGGGCTAGTGGCTCCGTCCGCTTCCACTTCTA27460.1921267224762378No Hit
GATCTATGCTGCGTCCACTTTGCAAAGTGGGGTCCCACCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA26990.18883831899612738No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26570.18589974567347553No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25140.1758946031701609No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24730.17302599587900075No Hit
GCATAGATCAGGAGTTCAGGGGCTTTCCCTGGTTTTTGCTGATACCAATT24420.1708570489027577No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24360.17043725271380744No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22960.16064200830496794No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22870.16001231402154253No Hit
GTCTCCATCCTCCCTGACTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCGTTAGCGC22870.16001231402154253No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22840.1598024159270674No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22650.1584730613287249No Hit
GGGTTCCAGTGCCCTTTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT22040.15420513340773057No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21730.1520361864314875No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG20820.14566927756574183No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19980.13979213092043813No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTC19940.1395122667944713No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19720.13797301410165366No Hit
GATCAGGAGTTCAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAAT19710.13790304807016193No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19370.13552420299944376No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19110.1337050861806593No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTCCAAAGGGCA18870.13202590142485823No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18790.13146617317292456No Hit
CTCCTGATCTATGCTGCGTCCACTTTGCAAAGTGGGGTCCCACCAAGGTT18630.13034671666905717No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC18230.12754807540938876No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGGTGGGACCCCA18140.12691838112596335No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18050.12628868684253794No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGGCACTTACTACTGTCAACAGGGTTCCAGTGCC17900.1252391963701623No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17280.12090130241767623No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT17000.11894225353590832No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16940.11852245734695806No Hit
GTCATGGACTTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC16470.11523405386684765No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16320.11418456339447199No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16300.11404463133148855No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16000.11194565038673723No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15930.11145588816629526No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15840.11082619388286988No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15730.11005656753646105No Hit
GTGGGGTCCCACCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT15160.10606850374143353No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15160.10606850374143353No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14930.10445928501712418No Hit
ACCATAGACAGCGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCAATAGTCCTGCGCCCCACTGCTGTAGCTGCAC14890.10417942089115734No Hit
GTTCAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAATAGGCGCTA14750.10319989645027339No Hit
GTAGTAAGTGCCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA14730.10305996438728998No Hit
CTGGAACCCTGTTGACAGTAGTAAGTGCCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC14610.10222037200938944No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC14590.10208043994640603No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14570.1019405078834226No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14530.10166064375745575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTGAT18800.056.24889870
GTGGTAT3850.048.4901731
TGGATTG19750.048.15299270
GACCGGA752.891882E-547.31450770
ATAAACG405.5352713E-542.391783
GTATCAA31800.040.134011
TAAAGGC501.1534394E-437.42541567
TATCAAC35350.035.706992
TCTACTG17550.035.24032668
ATCAACG36200.034.5692063
TCAACGC36250.034.5094454
GTGGATT20850.034.35249369
CAACGCA36900.033.9922455
AACGCAG37500.033.7195666
GTCTACT17550.033.4802867
AACTGTA850.003043129633.39847670
CGTGTAT552.442235E-432.94154764
TGTCTAC17550.032.88829866
CGACGCT18150.032.54051621
TCCGCGA18050.032.33676517
Produced by FastQC (version 0.11.5)