FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004686187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1193184
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGTCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG86810.7275491458148953No Hit
GACTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTATTCGG64460.5402352026175342No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC48810.4090735376941025No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCATTCAGGCTGTCATCCCATG48490.40639163783624316No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT42200.35367554375519616No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG42180.35350792501407996No Hit
GTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCC41010.34370222865878186No Hit
GTGTTACCTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT39300.3293708262933462No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC38250.32057084238474537No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT34750.2912375626894092No Hit
GTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC33690.2823537694102502No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCATTCAGG33340.2794204414407166No Hit
CTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCG33130.2776604446589964No Hit
AGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG33000.27657092284174106No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCT31700.26567570466918766No Hit
GAGGTAACACCGTGAGCTGGTATCAGCAGCTCCCAGGAACGGCCCCCAAA30330.25419382090272746No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG29620.24824335559310218No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28420.23818623112612974No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG27610.23139767211092338No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG27460.23014053155255182No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27350.22921862847641272No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC26670.22351959127846166No Hit
CTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGATACCAGCTCACGGTGTTACCTCCGATGTTGGAG26670.22351959127846166No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26640.22326816316678735No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA26530.2223462600906482No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26160.21924531337999836No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26140.21907769463888216No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25970.21765293533939442No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGA25900.2170662697454877No Hit
CCCCTGAGGGCCGCTGATTATCACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGATACCAGCTCA25740.21572531981655804No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG25040.20985866387749083No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCAC24870.20843390457800307No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24600.20617105157293425No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24070.20172915493335478No Hit
ATACCACACCATTCAGGCTGTCATCCCATGCTGCACAGTAATAGTCAGCC23930.20055582374554135No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23830.1997177300399603No Hit
GTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC23590.19770630514656584No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG23020.19292917102475396No Hit
GCTGATACCAGCTCACGGTGTTACCTCCGATGTTGGAGCTGCTTCCAGAA22580.18924155872019738No Hit
CATCTATAGTGATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC22420.18790060879126771No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA22100.18521870893340844No Hit
GATTATCACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGA21610.18111204977606135No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC21400.1793520529943412No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT21300.17851395928876018No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA21190.17759205621262103No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT21030.17625110628369137No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20940.17549682194866845No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19150.16049494461876793No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC18990.15915399468983826No Hit
GCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG18980.15907018531928016No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC18030.15110829511626034No Hit
GTCTGAGGATGAGGCTGACTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGAC17970.15060543889291172No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAC17970.15060543889291172No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC17790.14909687022286588No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT17770.14892925148174968No Hit
CAGTAATACAAGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGAAA17280.1448225923244026No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16990.14239212057821762No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16830.14105117064928796No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16670.1397102207203583No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC16620.13929117386756779No Hit
CCATTCAGGCTGTCATCCCATGCTGCACAGTAATAGTCAGCCTCATCCTC16420.1376149864564057No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAGGTAACACCGTGAGCTGGTATCAGCAGCTCCCAGGAACGGCCCC16290.13652546463915036No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16110.13501689596910452No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCATTCAGGC16080.1347654678574302No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15900.13325689918738434No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA15870.13300547107571004No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC15870.13300547107571004No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15860.13292166170515193No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15730.13183213988789658No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15720.13174833051733847No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15700.13158071177622227No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC15490.1298207149945021No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT15290.12814452758334005No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT15090.12646834017217798No Hit
ATCCCATGCTGCACAGTAATAGTCAGCCTCATCCTCAGACTGGAGCCCAC15060.12621691206050367No Hit
GTGATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG14950.12529500898436452No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC14950.12529500898436452No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT14770.12378644031431865No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGACTATTACTGTGCAG14680.12303215597929573No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG14650.12278072786762143No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14590.1222778716442728No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14480.12135596856813367No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC14390.12060168423311073No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG14320.12001501863920401No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14260.1195121624158554No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGCTGATACCAGCTCACGGTGTTACCT14230.11926073430418108No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC14140.11850644996915816No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC14060.11783597500469334No Hit
GCACAGTAATAGTCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAG14040.11766835626357713No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC13710.1149026470351597No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13150.11020932228390592No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC13130.11004170354278972No Hit
GTACAGACTGGGCCCAGGACCCTGCACAGTGAGTGAGGAGGGTGAGGAGG13130.11004170354278972No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG12880.10794646927883712No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG12690.10635409123823315No Hit
GTCAGGGACCCCTGAGGGCCGCTGATTATCACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGATA12550.10518076005041972No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC12530.10501314130930352No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT12510.1048455225681873No Hit
GCTTGGTCCCTCCGCCGAATACCACACCATTCAGGCTGTCATCCCATGCTGCACAGTAATAGTCAGCCTCATCCT12460.10442647571539679No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG12410.10400742886260628No Hit
GGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC12250.10266647893367661No Hit
GTGCAGCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGACC12240.10258266956311851No Hit
ATGCTGCACAGTAATAGTCAGCCTCATCCTCAGACTGGAGCCCACTGATG12200.1022474320808861No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG12000.10057124466972403No Hit
GATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG11990.10048743529916593No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG11960.10023600718749162No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)