FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1193184
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTATTCGG59400.497827661115134No Hit
GTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCC45400.38049454233378927No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC44370.3718621771663046No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG42880.3593745809531472No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG41150.34487555984659535No Hit
GTGTTACCTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT39760.33322605733901894No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCATTCAGGCTGTCATCCCATG38010.31855941749135086No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37470.3140337114812133No Hit
AGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG37410.31353085525786467No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37220.3119384772172607No Hit
GTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC37050.31051371791777294No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCT35460.29718802799903454No Hit
GAGGTAACACCGTGAGCTGGTATCAGCAGCTCCCAGGAACGGCCCCCAAA34560.2896451846488052No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT30190.25302048971491403No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC30100.25226620537989114No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG29970.2511766835626357No Hit
CTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCG29370.24614812132914957No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT29350.24598050258803336No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG28980.24287955587738355No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27770.23273862203985304No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCAC26650.22335197253734546No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26300.22041864456781185No Hit
GTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC26010.21798817282162683No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25860.2167310322632553No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25340.2123729449942339No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCATTCAGG25270.21178627940032718No Hit
CTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGATACCAGCTCACGGTGTTACCTCCGATGTTGGAG25080.21019390135972324No Hit
CATCTATAGTGATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC24700.20700914527851533No Hit
CCCCTGAGGGCCGCTGATTATCACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGATACCAGCTCA24010.20122629871000616No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23480.19678440207042666No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC22980.19259393354252152No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22880.1917558398369405No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22830.19133679298415No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG22600.18940917746131358No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22490.18848727438517446No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT22190.18597299326843136No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA22160.18572156515675706No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21300.17851395928876018No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21260.17817872180652775No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20830.1745749188725293No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20520.1719768283852281No Hit
GCTGATACCAGCTCACGGTGTTACCTCCGATGTTGGAGCTGCTTCCAGAA20180.1691273097862526No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC20100.16845683482178775No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAGGTAACACCGTGAGCTGGTATCAGCAGCTCCCAGGAACGGCCCC19750.16552350685225412No Hit
GATTATCACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGA19280.16158446643602328No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18920.15856732909593155No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT18690.1566397135730952No Hit
GTCTGAGGATGAGGCTGACTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGAC18620.15605304797918845No Hit
ATACCACACCATTCAGGCTGTCATCCCATGCTGCACAGTAATAGTCAGCC17990.15077305763402793No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC17310.14507402043607692No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17250.14457116421272828No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17230.14440354547161208No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT17210.14423592673049587No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC17060.14297878617212434No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA16730.14021307694370694No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16680.1397940300909164No Hit
GCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG16590.13903974575589348No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16380.1372797489741733No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC16370.1371959396036152No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT16060.134597849116314No Hit
GTGATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG16030.1343464210046397No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC16020.1342626116340816No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC15740.13191594925845468No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15590.13065880870008315No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC15460.1295692868828278No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15400.12906643065947918No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG15230.12764167135999144No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14940.12521119961380642No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT14850.1244569152787835No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGACTATTACTGTGCAG14530.12177501542092418No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAGGTAACACC14400.12068549360366883No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAC14160.11867406871027436No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGCTGATACCAGCTCACGGTGTTACCT14150.11859025933971626No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14110.11825502185748385No Hit
CAGTAATACAAGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGAAA14090.11808740311636765No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC14070.11791978437525144No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC14020.1175007375224609No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13810.11574074074074073No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG13800.11565693137018263No Hit
GTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAG13730.11507026577627591No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13620.11414836270013677No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13230.11087979724837074No Hit
GGTATCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGTGATAATCAGCGGCCCTCAGGGGTCC13210.11071217850725454No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC12990.10886837235497626No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12670.10618647249711695No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCATTCAGGC12490.1046779038270711No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC12340.10342076326869956No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC12330.10333695389814146No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC12310.10316933515702523No Hit
GGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC12090.10132552900474695No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTA12000.10057124466972403No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGA11950.10015219781693352No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAG11940.10006838844637542No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTAA150.00237576268.8515738
GTCTAGC150.002473836868.1527521
TAGCCCG150.00248083868.104195
GCTAGCT200.006870926452.6560653
GTATCAA31300.043.246871
TCTCGGG6050.040.9288370
TTAATCG451.0227438E-438.19746437
TATCAAC35700.037.9978752
ATCAACG38050.035.540653
TCAACGC38200.035.3907054
CAACGCA39050.034.6189045
AACGCAG40000.033.9669656
ATAGCGG552.6400192E-432.50686363
CGCAGAG42300.032.120068
ACGCAGA42400.032.0443087
AGAGTAC42700.031.73809411
AATCGTT553.2821094E-431.32271439
TAATCGT553.298467E-431.29617338
CAGAGTA43300.031.21966710
GCAGAGT43350.031.1849639
Produced by FastQC (version 0.11.5)