FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1444175
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAATATGGTAGTTCACGTCGGACGTTCGGCCAAGGGA64170.4443367320442467No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42400.2935932279675247No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA41640.2883307078435785No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35580.24636903422369175No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA34230.23702113663510307No Hit
CTCCTGTAGGGCCAGTCAGAGTGTTAGTAGTAGCTTCTTAGCCTGGTTTC30750.21292433396229682No Hit
CTATACAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACAGCAAAAACTCCCTGTATCTGCAAATGAA29630.2051690411480603No Hit
GTGTATTACTGTCAGCAATATGGTAGTTCACGTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA29220.20233005002856302No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28080.19443626984264373No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27380.1895892118337459No Hit
GTAATACAAGGCGGTGTCCTCAGGTCTCAGACTGTTCATTTGCAGATACA26200.181418456904461No Hit
GTATTACTGTGCAAACCTACCTACCACCTTTGATGTTTTTGATATCTGGGGCCAAGGGACAATGGTCATCGTCT25030.17331694566101755No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23630.1636228296432219No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA23490.16265341804144234No Hit
CAGTAATACAAGGCGGTGTCCTCAGGTCTCAGACTGTTCATTTGCAGATA23410.16209946855471116No Hit
GTCTGAGACCTGAGGACACCGCCTTGTATTACTGTGCAAACCTACCTACC23230.160853082209566No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23020.15939896480689666No Hit
GTGTTAGTAGTAGCTTCTTAGCCTGGTTTCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAT22420.15524434365641282No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22090.15295930202364671No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGATTCAGTGGCAGTGGGTCTGGGACAGACTTCA22080.15289005833780533No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21860.15136669724929458No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21740.15053577301919782No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21530.14908165561652847No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT21290.14741980715633493No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT20890.14465005972267905No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20770.14381913549258227No Hit
GTCCCAGACCCACTGCCACTGAATCTGTCTGGGATGCCAGTGGCCCTGCT20290.14049543857219518No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20190.1398030017137812No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20130.13938753959873285No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20060.13890283379784307No Hit
GAGTTTGGACTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGT19290.13357106998805546No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19250.13329409524468988No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19030.13177073415617915No Hit
GTCTGTATAGTATGTACTACCACCATCCCAACTAATAAGAGAGACCCACT19010.13163224678449634No Hit
TGATTATACCATGCACTGGGTCCGTCAAGTTCCGGGGAAGGGTCTGGAGT18920.13100905361192378No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGAAACCAGGCTAAGAAGCTACTACTAACACTCTGA18870.13066283518271676No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18540.12837779354995066No Hit
GTATAGTATGTACTACCACCATCCCAACTAATAAGAGAGACCCACTCCAGACCCTTCCCCGGAACTTGACGGACC18320.12685443246143993No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTGATGATTATACCATGCACTGGGTCCGTCAAGTTCCGGGGAAGGGT17810.12332300448352866No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGA17570.12166115602333513No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT17560.12159191233749372No Hit
ATACTATACAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACA17380.12034552599234857No Hit
GCACTGAACACAGAGAACGCACCATGGAGTTTGGACTGAGCTGGGTTTTC17350.12013779493482439No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCAGGTGTTTCCATTCGGTGAT17180.11896065227552062No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC16650.11529073692592656No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC16650.11529073692592656No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAATATGGTAGTTCA16350.11321342635068465No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC16270.11265947686395347No Hit
CCATTGTCCCTTGGCCCCAGATATCAAAAACATCAAAGGTGGTAGGTAGGTTTGCACAGTAATACAAGGCGGTGT16210.11224401474890508No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAATCTGTCTGGGATGCCA16070.11127460314712552No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT16040.11106687208960132No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15780.10926653625772499No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15690.10864334308515243No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGT15480.10718922568248308No Hit
CTCCAGACCCTTCCCCGGAACTTGACGGACCCAGTGCATGGTATAATCAT15180.10511191510724116No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15150.10490418404971696No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15100.10455796562050998No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14790.10241141135942666No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14740.10206519293021968No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGATGACCATTGTCCCTTGG14480.10026485709834333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT309.215921E-657.4061339
TCGTATG5100.044.6226740
TAGCGCT7150.043.89593529
ACTAGCG7000.043.81165727
CGTATGC5600.043.7755641
CTAGCGC7250.042.7991228
ATGCCGT5300.042.5180144
ATCTCGT4950.041.64188437
CTTATAC7550.041.4551661
GCCGTCT5300.041.33666246
ATGTCGT503.956702E-641.22546437
GACTAGC7550.041.05005626
CACGAGA7400.040.9247821
CGAGACT7650.040.48398223
ACGAGAC7750.039.9574622
CGCTCAT7200.039.89755232
GCGCTCA7750.039.67502231
TCTCCGA7900.039.6130611
TATACAC8000.039.5485653
GTATGCC6450.039.13781742
Produced by FastQC (version 0.11.5)